Improved growth and survival of cultured neural cell populations

Light microscopic picture of neurosphere formation after seven days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (P) and magnetically isolated using Anti-Prominin-1 MicroBeads prior to cell culture.

Fluorescence microscopy image of PSA-NCAM–positive neuronal precursor cells after seven days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti-mouse Alexa Flour™ 594 (red), a marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).
 

Fluorescence image of A2B5-positive glial precursor cells after seven days of cultivation in MACS Neuro Medium supplemented with MACS NeuroBrew-21. A2B5-positive cells were processed for immunofluorescent detection of anti-GFAP antibody and goat anti-rabbit AF-488 (green), a marker for glial precursor cells.