IgG
⁺
Memory B Cell Isolation Kit
, human

Name: IgG+ Memory B Cell Isolation Kit, human
Availability: InStock

IgG
⁺
Memory B Cell Isolation Kit
, human

The IgG

⁺

Memory B Cell Isolation Kit has been developed for the isolation of IgG

⁺

memory B cells from human PBMCs.

Data and images
Specifications

Product data and images for IgG
⁺
Memory B Cell Isolation Kit
, human

Figures

Figure 1

IgG

⁺

memory B cells were isolated from human PBMCs using the IgG

⁺

Memory B Cell Isolation Kit. Cells were fluorescently stained with Anti-IgG-APC and CD19-PE.

Unseparated fraction	Pre-enriched memory B cells
View details IgG ⁺ Memory B Cell Isolation Kit , human Figure 1 IgG ⁺ memory B cells were isolated from human PBMCs using the IgG ⁺ Memory B Cell Isolation Kit. Cells were fluorescently stained with Anti-IgG-APC and CD19-PE.	View details IgG ⁺ Memory B Cell Isolation Kit , human Figure 1 IgG ⁺ memory B cells were isolated from human PBMCs using the IgG ⁺ Memory B Cell Isolation Kit. Cells were fluorescently stained with Anti-IgG-APC and CD19-PE.
Isolated IgG+ memory B cells
View details IgG ⁺ Memory B Cell Isolation Kit , human Figure 1 IgG ⁺ memory B cells were isolated from human PBMCs using the IgG ⁺ Memory B Cell Isolation Kit. Cells were fluorescently stained with Anti-IgG-APC and CD19-PE.

Specifications for IgG
⁺
Memory B Cell Isolation Kit
, human

Overview

The IgG

⁺

Memory B Cell Isolation Kit has been developed for the isolation of IgG

⁺

memory B cells from human PBMCs.

Detailed product information

Background information

Memory B cells, defined as quiescent antigen-experienced B cells, are generated in response to T cell–dependent and T cell–independent antigens. They are able to react quickly to a recurrent antigenic challenge thereby providing serological immune protection.

Detailed separation procedure

IgG

⁺

memory B cells are isolated by depletion of unwanted cells and subsequent positive selection with Anti-IgG MicroBeads. Non-B cells (T cells, NK cells, monocytes, dendritic cells, granulocytes, platelets, and erythroid cells) are labeled with a cocktail of biotinylated CD2, CD14, CD16, CD36, CD43, and CD235a (Glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads.

Columns

For the first magnetic separation (depletion): LD or autoMACS

^®

Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Data and images