Clone:
REA1215
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
CSF2

Extended validation for GM-CSF Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1215
BVD2-21C11++
DAVKAT-
Cells were incubated with an excess of purified unconjugated GM-CSF (REA1215) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for GM-CSF. Human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA & Ionomycin and were stained with GM-CSF antibodies and with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with (specificity from PIM) antibodies. As a control, GM-CSF antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GM-CSF. Human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA & Ionomycin and were stained with GM-CSF antibodies and with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with (specificity from PIM) antibodies. As a control, GM-CSF antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GM-CSF. Human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA & Ionomycin and were stained with GM-CSF antibodies and with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with (specificity from PIM) antibodies. As a control, GM-CSF antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GM-CSF. Human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA & Ionomycin and were stained with GM-CSF antibodies and with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with (specificity from PIM) antibodies. As a control, GM-CSF antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for GM-CSF. Human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA & Ionomycin and were stained with GM-CSF antibodies and with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with (specificity from PIM) antibodies. As a control, GM-CSF antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for GM-CSF. Human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA & Ionomycin and were stained with GM-CSF antibodies and with a suitable counterstaining. Cells were then fixed and permeabilized using the Inside Stain Kit followed by a staining with (specificity from PIM) antibodies. As a control, GM-CSF antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and Viobility 405/520 Fixable Dye fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

Specifications for GM-CSF Antibody, anti-human, REAfinity™

Overview

Clone REA1215 recognizes the human granulocyte macrophage colony-stimulating factor (GM-CSF), which is a hematopoietic growth factor that is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Additional information: Clone REA1215 displays negligible binding to Fc receptors.

Alternative names

CSF2

Detailed product information

Technical specifications

CloneREA1215
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman, non-human primate
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
AntigenGM-CSF
Alternative names of antigenCSF2
Molecular mass of antigen [kDa]14
Distribution of antigenT cells, endothelial cells, fibroblasts, macrophages, other
Entrez Gene ID1437
RRIDAB_2811515, AB_2811520, AB_2811516, AB_2811521, AB_2811517, AB_2811522, AB_2811518, AB_2922022, AB_2922005, AB_2811519

Resources for GM-CSF Antibody, anti-human, REAfinity™

Certificates

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