CD4 (TIL) MicroBeads, mouse

CD4 (TIL) MicroBeads, mouse have been optimized for the isolation of CD4
+
tumor-infiltrating lymphocytes from mouse tumors.

Data and images for CD4 (TIL) MicroBeads, mouse

Figures

Figure 1

A tumor induced by the B16-F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4
+
TILs were isolated from the single-cell suspension using CD4 (TIL) MicroBeads, optional wash step, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE and CD3ε-APC-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and DAPI fluorescence.
Before separation
CD4
+
cells
View details

Figure 1

A tumor induced by the B16-F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4
+
TILs were isolated from the single-cell suspension using CD4 (TIL) MicroBeads, optional wash step, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE and CD3ε-APC-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and DAPI fluorescence.
View details

Figure 1

A tumor induced by the B16-F10 cell line was dissociated using the gentleMACS™ Octo Dissociator with Heaters in combination with the Tumor Dissociation Kit, mouse. CD4
+
TILs were isolated from the single-cell suspension using CD4 (TIL) MicroBeads, optional wash step, two MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD4-PE and CD3ε-APC-Vio
®
770 and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and DAPI fluorescence.

Specifications for CD4 (TIL) MicroBeads, mouse

Overview

CD4 (TIL) MicroBeads, mouse have been optimized for the isolation of CD4
+
tumor-infiltrating lymphocytes from mouse tumors.

Detailed product information

Background information

CD4 (TIL) MicroBeads, mouse enable a positive selection of CD4
+
lymphocytes from solid mouse tumors, e.g., CT26.WT, 4T1, and B16F10 and have specifically been developed for an optimal isolation of tumor-infiltrating lymphocytes (TILs) from mouse tumors. The CD4 antigen is expressed on T helper cells, regulatory T cells, and at lower levels on subpopulations of NKT cells and dendritic cells.

Columns

MS, LS, autoMACS
®
Columns, or Multi-24 Column Blocks

Resources for CD4 (TIL) MicroBeads, mouse

Documents and Protocols

Scientific posters

Optimized workflow improves the characterization of tumor-infiltrating T cells

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