CD36 Antibody, anti-human

Name: CD36 Antibody, anti-human
Availability: InStock

CD36 Antibody, anti-humanExtended Validation

Clone:

AC106

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2aκ, mouse IgG2a

Applications:

FC, MICS, IF, IHC, MC

Alternative names:

BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Scarb3, gpIIIb

Flow cytometry applications forCD36 Antibody, anti-human

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
PerCP
PerCP-Vio 700
VioBlue
VioGreen

Conjugate:

APC

Recommended dilution:

1:11

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Products used:

130-100-307, 130-095-475

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies as well as with Anti-HLA-DR antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD36 Antibody, anti-human

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:11

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-100-307, 130-095-475

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:11

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-100-307, 130-095-475

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Mass cytometry applications forCD36 Antibody, anti-human

pure

Conjugate:

pure

Products used:

130-108-018

Description:

Human peripheral blood cells after erythrocyte lysis were stained with 141Pr-CD45 and 166Er-CD36 antibodies. Data courtesy: Dr. Sabine Baumgart and Anette Peddinghaus, DRFZ, Germany

Extended validation for CD36 Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with AC106
5-271	++
CB38	++
CLB-IVC7	++
NL07	++
REA760	++
Cells were incubated with an excess of purified unconjugated CD36 (AC106) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Flow cytometric comparison of different clones for CD36. Human peripheral blood mononuclear cells (PBMCs) were stained with CD36 antibodies and with a suitable counterstaining. As a control, CD36 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD36 (AC106). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD36 (AC106). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD36 Antibody, anti-human

Overview

CD36 is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, monocytes, macrophages, and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor for long chain fatty acids, oxidized LDL, collagen type I, IV, and V and thrombospondin, as well as for apoptotic cells. CD36 is an early marker of erythroid differentiation.

Alternative names

BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Scarb3, gpIIIb

Detailed product information

Technical specifications

Clone	AC106
Clonality	monoclonal
Isotype	mouse IgG2a, mouse IgG2aκ
Isotype control	Isotype Control Antibody, mouse IgG2a
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD36
Alternative names of antigen	BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, Scarb3, gpIIIb
Molecular mass of antigen [kDa]	53
Distribution of antigen	B cells, red blood cells, dendritic cells, endothelial cells, macrophages, monocytes, platelets, basophils
Entrez Gene ID	948
RRID	AB_2661001, AB_2660990, AB_10827705, AB_2660991, AB_10829944, AB_2660992, AB_10828457, AB_2660993, AB_2660994, AB_10830713, AB_2660995, AB_2660996, AB_2660999, AB_2661000, AB_10827691, AB_2751985, AB_2751646, AB_2751600, AB_2752015

Resources for CD36 Antibody, anti-human

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

Reviews for CD36 Antibody, anti-human

Very Good CD36 Antibody from Miltenyi Biotec

1
2
3
4
5

CD36-FITC, human (130-120-064)

Will buy and use it again. Recommendable!

Write a review

powered by

Applications

Extended validation