CD34
⁺
CD38
^-
Cell Isolation Kit
, human

Name: CD34+CD38- Cell Isolation Kit, human
Availability: InStock

CD34
⁺
CD38
^-
Cell Isolation Kit
, human

The CD34

⁺

CD38

^–

Cell Isolation Kit, human has been developed to isolate a CD38

^–

subset of CD34

⁺

cells, i.e., primitive hematopoietic stem and progenitor cells, from cord blood, bone marrow, or apheresis.

Product data and images for CD34
⁺
CD38
^-
Cell Isolation Kit
, human

Figures

Figure 1

CD34

⁺

CD38

^–

cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue

^®

, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	Before CD38 depletion
View details CD34 ⁺ CD38 ^- Cell Isolation Kit , human Figure 1 CD34 ⁺ CD38 ^– cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue ^® , Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details CD34 ⁺ CD38 ^- Cell Isolation Kit , human Figure 1 CD34 ⁺ CD38 ^– cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue ^® , Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
CD34 ⁺ CD38 ^– cells	CD34 ⁺ CD38 ⁺ cells
View details CD34 ⁺ CD38 ^- Cell Isolation Kit , human Figure 1 CD34 ⁺ CD38 ^– cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue ^® , Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details CD34 ⁺ CD38 ^- Cell Isolation Kit , human Figure 1 CD34 ⁺ CD38 ^– cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue ^® , Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD34
⁺
CD38
^-
Cell Isolation Kit
, human

Overview

The CD34

⁺

CD38

^–

Cell Isolation Kit, human has been developed to isolate a CD38

^–

subset of CD34

⁺

cells, i.e., primitive hematopoietic stem and progenitor cells, from cord blood, bone marrow, or apheresis.

Detailed product information

Background information

The CD34

⁺

CD38

^–

Cell Isolation Kit has been developed for the isolation of the more primitive CD38

^–

subpopulation of CD34

⁺

cells. CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts, endothelial progenitor cells, and mature endothelial cells. CD38 is absent on most primitive CD34

⁺

stem cells and upregulated on CD34

⁺

cells upon lymphocyte commitment. CD34

⁺

CD38

^-

cells are isolated in a two-step isolation protocol. First, the CD34

⁺

cells are positively enriched using CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34

⁺

are then labeled with CD38 MicroBeads to deplete CD38

⁺

cells in a second separation step.

Downstream applications

CD34⁺ CD38^– cell expansion in culture
Lentiviral transduction
Molecular analysis
In vitro differentiation of CD34⁺ CD38^– cells
Generation of humanized mice

Columns

LS Columns.

Resources for CD34
⁺
CD38
^-
Cell Isolation Kit
, human

Documents and Protocols

Brochures/posters

Solutions for cutting-edge human hematopoietic stem cell research

Scientific posters

Efficient isolation of human hematopoietic stem cells using immunomagnetic sorting

Data and images