CD24 Antibody, anti-human

Name: CD24 Antibody, anti-human
Availability: InStock

CD24 Antibody, anti-humanExtended Validation

Clone:

32D12

Type of antibody:

Primary antibodies

Isotype:

mouse IgG1κ, mouse IgG1

Applications:

FC, MICS, IF, IHC, MC

Alternative names:

CD24A, BA-1, HAS

Flow cytometry applications forCD24 Antibody, anti-human

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
PerCP-Vio 700
VioBlue

Conjugate:

APC

Recommended dilution:

1:11

Remark:

The antibody is suited for staining of formaldehyde-fixed cells.

Tested:

QC tested

Reported:

26884312, 27432228,

Products used:

130-099-399, 130-095-954

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:11)

Description:

White blood cells (WBCs) were stained with CD24 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD24 Antibody, anti-human

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:11

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-099-399, 130-095-954

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Mass cytometry applications forCD24 Antibody, anti-human

pure

Conjugate:

pure

Products used:

130-108-037

Extended validation for CD24 Antibody, anti-human

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD24. Human peripheral blood mononuclear cells (PBMCs) were stained with CD24antibodies and with a suitable counterstaining. As a control, CD24 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD24. Human peripheral blood mononuclear cells (PBMCs) were stained with CD24antibodies and with a suitable counterstaining. As a control, CD24 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD24. Human peripheral blood mononuclear cells (PBMCs) were stained with CD24antibodies and with a suitable counterstaining. As a control, CD24 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD24. Human peripheral blood mononuclear cells (PBMCs) were stained with CD24antibodies and with a suitable counterstaining. As a control, CD24 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD24. Human peripheral blood mononuclear cells (PBMCs) were stained with CD24antibodies and with a suitable counterstaining. As a control, CD24 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD24. Human peripheral blood mononuclear cells (PBMCs) were stained with CD24antibodies and with a suitable counterstaining. As a control, CD24 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD24 (32D12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD24 (32D12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD24 Antibody, anti-human

Overview

The human CD24 antigen is also known as heat-stable antigen (HSA). CD24 has been identified to be a negative marker for breast cancer stem cells and a positive marker for ovarian or pancreatic cancer stem cells. The CD24 antibody can be used, for example, to differentiate CD44

⁺

CD24

^–

breast cancer stem cells from CD24

⁺

expressing cells from a primary tumor sample.

Alternative names

CD24A, BA-1, HAS

Detailed product information

Technical specifications

Clone	32D12
Clonality	monoclonal
Isotype	mouse IgG1κ, mouse IgG1
Isotype control	Isotype Control Antibody, mouse IgG1
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD24
Alternative names of antigen	CD24A, BA-1, HAS
Molecular mass of antigen [kDa]	3
Distribution of antigen	B cells, stem cells, endothelial cells, epithelial cells, granulocytes, Langerhans cells, monocytes, ILC, red blood cells, leukemia cells, ES and iPS cells, thymocytes, cancer stem cells, breast, lung, ovary, pancreas, B cells, endothelial cells, epithelial cells, granulocytes, Langerhans cells, monocytes, red blood cells, leukemia cells, transitional B cells, breast, lung, ovary, pancreas
Entrez Gene ID	100133941
RRID	AB_2751980, AB_2904867, AB_2660668, AB_2921773, AB_2904870, AB_2660657, AB_10829613, AB_2660660, AB_2660661, AB_2660666, AB_2660667, AB_2904866, AB_2904865, AB_2904869, AB_2904868, AB_2752010

Resources for CD24 Antibody, anti-human

Documents and Protocols

Brochures/posters

Cancer Research - Understanding cancer begins with the cell

Scientific posters

Magnetic isolation and rare cell gene expression analysis of breast cancer stem cells

Scientific posters

Isolation of tumor cell subpopulations using semi-automated tissue dissociation and magnetic cell separation

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CD24 Antibody, anti-human

Publications

Al-Hajj, M. et al. (2003) Prospective identification of tumorigenic breast cancer cells. Proc. Natl. Acad. Sci. U.S.A. 100: 3983-3988
Li, C. P. et al. (2007) Identification of pancreatic cancer stem cells. Cancer Res. 67: 1030-1037
Gao, M. Q. et al. (2010)
CD24
⁺
cells from hierarchically organized ovarian cancer are enriched in cancer stem cells.
Oncogene 29: 2672-2680

Applications

Extended validation