CD19 MicroBeads, mouse

CD19 MicroBeads, mouse, were developed for positive selection or depletion of mouse B cells from single-cell suspensions of spleen.

Data and images for CD19 MicroBeads, mouse

Figures

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD19
+
B cells were isolated from this single-cell suspension using the CD19 MicroBeads, and autoMACS
®
Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45R (B220)-APC-Vio
®
770 (clone REA755), mouse, and CD19-PE-Vio770, mouse (clone REA749) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals and 7-AAD Staining Solution fluorescence.
Before separation
After separation
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD19
+
B cells were isolated from this single-cell suspension using the CD19 MicroBeads, and autoMACS
®
Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45R (B220)-APC-Vio
®
770 (clone REA755), mouse, and CD19-PE-Vio770, mouse (clone REA749) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals and 7-AAD Staining Solution fluorescence.
View details

Figure 1

A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD19
+
B cells were isolated from this single-cell suspension using the CD19 MicroBeads, and autoMACS
®
Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45R (B220)-APC-Vio
®
770 (clone REA755), mouse, and CD19-PE-Vio770, mouse (clone REA749) and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals and 7-AAD Staining Solution fluorescence.

Specifications for CD19 MicroBeads, mouse

Overview

CD19 MicroBeads, mouse, were developed for positive selection or depletion of mouse B cells from single-cell suspensions of spleen.

Detailed product information

Background information

The mouse monoclonal antibody reacts with mouse CD19, which is expressed on B cell lineage cells, throughout their development from early pro-B cell stages on, and is down-regulated upon terminal differentiation to plasma cells. Mouse CD19 MicroBeads are used for positive selection or depletion of mouse B cells from various tissues such spleen.

Downstream applications

  • Positive selection or depletion of CD19+ B cells spleen.
  • Isolation of CD19+ B cells to study the accessibility of enhancer elements during B cell development by in vivo footprinting.
  • Isolation of pure B cells for preparation of cell extracts to analyze conditions for V(D)J recombination in a cell-free system.

Columns

For positive selection: LS or autoMACS
®
Columns. For depletion: LD or autoMACS Columns.

Resources for CD19 MicroBeads, mouse

Documents and Protocols

Brochures/posters

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