CD11c Antibody, anti-human

Name: CD11c Antibody, anti-human
Availability: InStock

CD11c Antibody, anti-humanExtended Validation

Clone:

MJ4-27G12

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2b

Applications:

FC, MICS, IF, IHC, MC

Alternative names:

ITGAX, CR4, SLEB6, 95, integrin αX, p150

Flow cytometry applications forCD11c Antibody, anti-human

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
PerCP-Vio 700
VioBlue
pure

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Reported:

23783831, 27622063,

Products used:

130-114-102, 130-113-576

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies or with the corresponding isotype control antibodies (left images) as well as with CD1c (BDCA-1) antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD11c Antibody, anti-human

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-114-102, 130-113-576

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Mass cytometry applications forCD11c Antibody, anti-human

pure

Conjugate:

pure

Products used:

130-108-030

Extended validation for CD11c Antibody, anti-human

Specificity

Epitope competition

In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.

Other clones	Overlap in epitope recognition with MJ4-27G12
S-HCL-3	-
3.9	-
Bu15	-
B-ly6	-
REA618	-
Cells were incubated with an excess of purified unconjugated CD11c (MJ4-27G12) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD11c. Human peripheral blood mononuclear cells (PBMCs) were stained with CD11c antibodies and with a suitable counterstaining. As a control, CD11c antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD11c (MJ4-27G12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD11c (MJ4-27G12). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD11c Antibody, anti-human

Overview

This CD11 antibody reacts with human CD11c, a 145–150 kDa type I transmembrane glycoprotein, which is also known as integrin αX or CR4. It is expressed on monocytes, macrophages, NK cells, granulocytes, myeloid dendritic cells (MDCs), and subsets of T and B cells.

On myeloid dendritic cells, CD11c is highly expressed on type 1 myeloid dendritic cells (CD1c (BDCA-1)

⁺

CD123

^low

MDC1s) and low on type 2 myeloid dendritic cells (CD1c (BDCA-1)

^-

CD123

^-

MDC2s).

CD11c, also known as integrin integrin αX or CR4, has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.

Alternative names

ITGAX, CR4, SLEB6, 95, integrin αX, p150

Detailed product information

Technical specifications

Clone	MJ4-27G12
Clonality	monoclonal
Isotype	mouse IgG2b
Isotype control	Isotype Control Antibody, mouse IgG2b
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD11c
Alternative names of antigen	ITGAX, CR4, SLEB6, 95, integrin αX, p150
Molecular mass of antigen [kDa]	126
Distribution of antigen	B cells, T cells, NK cells, dendritic cells, granulocytes, monocytes, macrophages, leukemia cells, B cells, dendritic cells, granulocytes, lymphocytes, macrophages, monocytes, myeloid leukemia cells, NK cells, T cells, leukemia cells
Entrez Gene ID	3687
RRID	AB_2726179, AB_2726457, AB_2726180, AB_2726453, AB_2726176, AB_2726460, AB_2726183, AB_2726458, AB_2726181, AB_2726454, AB_2726177, AB_2726459, AB_2726182, AB_2726455, AB_2726178, AB_2660150, AB_871586, AB_2726456

Resources for CD11c Antibody, anti-human

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

References for CD11c Antibody, anti-human

Publications

Dzionek, A. et al. (2000) BDCA-2, BDCA-3, BDCA-4: Three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol 165: 6037-6046
Bendiss-Vermare, N. et al. (2001)
Human thymus contains IFN-alpha-producing CD11c
^–
, myeloid CD11c
⁺
, and mature interdigitating dendritic cells.
J. Clin. Invest. 107: 835-844
Barclay, A. N. et al. (1997) In: The Leukocyte Antigen Facts Book, Academic Press, San Diego, CA. (2nd edition): 161-162

Applications

Extended validation