| Principle | Any positive and untouched isolation can be performed to separate target cells from PBMCs, cultured cells, and dissociated tissue. In addition, cells can be directly isolated from whole blood or bone marrow using StraightFrom® Whole Blood MicroBeads. Overall more than 400 different MicroBeads and Isolation Kits are available and we are steadily expanding our portfolio. Upon automated separation, labeled cells as well as non-labeled cells are obtained and can be used for a variety of downstream applications such as functional and molecular assays, chimerism analysis, and flow cytometry. To ensure cell viability and integrity, cells are kept cooled in Chill Racks. |
|---|---|
| Monitoring | Pre-set programs for all MACS® MicroBeads and Isolation Kits ensure accurate sample handling. Sensors constantly monitor the status of the autoMACS® Pro, buffer levels, and column status. This ensures optimal instrument performance and safety of samples and operators. |
| Level of automation | Fully automated |
| Footprint | Without MiniSampler: 605x343 mm (W x D) With MiniSampler: 605x455 mm (W x D) Height: 392.5-454 mm (adjustable touchscreen) It is a benchtop-sized instrument that will easily fit under a common laminar flow hood. |
| Order number | 130-092-545 |
| Characteristics | The reusable autoMACS column is specifically designed for use with the autoMACS Pro Separator delivering high cell purities and recoveries. The column is compatible with all starting materials and available MicroBeads and Isolation Kits. One or two columns can be installed and thus increase the flexibility with regards to high recovery or high purity processes. Once installed, the columns become part of a closed fluidic system that keeps you safe when dealing with hazardous samples. Monitoring and priming of the column are automatically managed by the instrument software. |
|---|---|
| Capacity | - Up to 4 x 109 total cells/sample - Up to 2 x 108 magnetically labeled cells/sample - Up to 15 mL of whole blood |
Separation strategies
| Positive Separation | MACS MicroBeads StraightFrom® Whole Blood MicroBeads MACSprep™ Chimerism and Multiple Myeloma |
|---|---|
| Untouched isolation | MACS Isolation Kits (e.g. CD4+ T Cell Isolation Kit, human; Dead Cell Removal Kit) |
| Indirect MicroBeads | e.g. Anti-Biotin MicroBeads |
Starting material
| Sample type | - PBMCs - Whole blood - Bone marrow - Single-cell suspensions from dissociated tissue or tumor - Cultured cells (cell lines and primary cells) |
|---|---|
| Species | - Human - Mouse - Rat - Non-human primate - any others are possible with indirect MicroBeads |
| Number of samples | - 6 samples with 5 mL tubes - 5 samples with 15 mL tubes - 3 samples with 50 mL tubes |
| Volume | 0.2 mL - 50 mL sample volume depending on MicroBeads used |
Consumables
Accessories
Brochures and posters
Scientific posters
- Gene expression analysis reveals mechanisms with potential relevance to NK cell expansion protocols for immunotherapy
- Novel methods for the isolation of tumor cells from human, mouse, and xenografted tumors
- T helper cells a complete workflow for cell preparation, isolation, stimulation, polarization, and analysis
- High-purity enrichment of human hematopoietic stem cells utilizing MACS® Technology
- An in vitro method for the identification of DNP- and TNP-specific T cells in the naive compartment of human blood
- Flow cytometric multicolor NK cell phenotyping as a potential tool for donor selection and NK cell reconstitution analysis
- Workflow automation and parallelization improves the isolation and analysis of tumor-infiltrating immune cell subpopulations
- Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol. Zhang, H. et al. SfN (2016)
- Gene expression analysis of CD14+ monocytes immunomagnetically separated directly from whole blood: adaptation of protocols towards clinical trial requirements
Customer reports and application notes
- Isolation of monocytes with high purity directly from whole blood for transcriptome analysis in translational research. Foucher et al. (2013) MACS&more 15(1)
- CD56+ cell depletion from human PBMCs applied to validate an HIV-specific ADCC mechanism. Bardhi. A. (2017)
- Effector memory T helper cells secrete IFN-γ upon stimulation with cytokines: a role in chronic inflammation. Sattler et al. (2011) MACS&more 13(1).
- An automated method for purification of CD138+ cells from whole bone marrow samples for multiple myeloma research. Mossafa and Defasque (2013) MACS&more 15(1)
- MACS® Technology facilitates mixed lymphocyte reaction
- Measuring prolyl aminopeptidase activity in extracts prepared from magnetically purified malaria parasites. da Silva et al. (2013) MACS&more 15(1).
- Willasch et al. (2012) Enrichment of cell subpopulations by MACS® Technology for PCR-based chimerism analysis. MACS&more 13(1).
- Generation of purified human iPSC derived cardiomyocytes using clinically relevant workflows. Herron, T. (2017)
- autoMACS Pro vs. FACS
- CCR4 – a potential marker for the selective removal of effector-type FOXP3+CD4+ regulatory T cells in cancer immunotherapies. Sugiyama & Nishikawa (2018)
- autoMACS Pro and Multiple Myeloma Research
- Allogenic T cell infusion alters iron regulatory gene expression. Deeg, H.J. (2018)
- Modulation of allergic airway inflammation by the histamine H4 receptor: analysis by flow cytometry. Hartwig et al. (2012) MACS&more 14(1).
- Purification of YSD antibody libraries using the autoMACS® Pro Separator. Miller K.D. et al. (2012)
- Generation of functional CAR T cells from pan T cells isolated by MACS® technology
- Generation of functional CAR T cellsfrom CD4+ and CD8+ T cells
- Mouse NK cells isolated to high purity by MACS® Technology are fully functional. Meinhardt et al. (2013) MACS&more 15(1).
- Best practices in optimizing sample processing for improved flow cytometry. Spring C. (2017)
- Purification of human hematopoietic cells from xenografts for the assessment of functional and genomic identity. Dobson, S. et al. (2015)
- TCR clonotype sequence is only one factor defining the antiviral capacity of HIV-specific CD8+ T cells. Flerin, N. (2018)
- Depletion of mouse cells from dissociated patient derived xenograft tumors using the gentleMACS™ OctoDissociator with Heaters, followed by magnetic cell separation. Moro, M. (2014)
Other resources
Publications
- Ferretti, A. et al. (2020) Unbiased Screens Show CD8+ T Cells of COVID-19 Patients Recognize Shared Epitopes in SARS-CoV-2 that Largely Reside outside the Spike Protein. Immunity. 53(5): 1095-1107
- Saini S. et al. (2020) Human endogenous retroviruses form a reservoir of T cell targets in hematological cancers. Nat Commun. 11:5660
- Akio T. et al. (2020) Activated invariant natural killer T cells directly recognize leukemia cells in a CD1d-independent manner. Cancer Sci. 111(7): 2223-2233
- Gabr H. et al. (2020) CRISPR-mediated gene modification of hematopoietic stem cells with beta-thalassemia IVS-1-110 mutation. Stem Cell Res Ther. 11: 390
- Hasegawa, N. et al. (2019) Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter. Sci Rep 9: 20047
- Kulis, M. et al. (2015) Whole‑genome fingerprint of the DNA methylome during human B cell differentiation. Nat. Genet. 47(7): 746-756
- Haus, D. L. et al. (2014) CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice Stem Cell Res. 13(2): 214-226
- Kim, MS. et al. (2014) A draft map of the human proteome. Nature 509(7502): 575-581
- Kuwahara, M. et al. (2014) The Menin-Bach2 axis is critical for regulating CD4 T-cell senescence and cytokine homeostasis Nat Commun 2(5): 3555
- Riquelme, P. et al. (2013) IFN-γ-induced iNOS expression in mouse regulatory macrophages prolongs allograft survival in fully immunocompetent recipients Mol. Ther. 21(2): 409-422
- Bénéteau, M. et al. (2012) Combination of glycolysis inhibition with chemotherapy results in an antitumor immune response Proc. Natl. Acad. Sci. U.S.A. 109(49): 20071-20076
The autoMACS Pro Separator is highly time-saving, while providing a fast, reproducible, and cell friendly cell separation. With small modifications, even better purities can be obtained compared to manual separation.
Experimental Hematology Research Group
Clinic and Policlinic for Pediatrics, University Medical Center Carl Gustav Carus, Dresden
The research group uses their autoMACS Pro Separator for the isolation of human and murine HSCs, T cells, and dendritic cells. They perform both positive and negative selection strategies with important markers such as BDCA2, BDCA4, CD4, CD8, CD11b, CD105 and a lineage-panel. After cell separation, cells are used for in vivo (e.g. transplantation) and in vitro (e.g. co-culture, stimulation, and analysis) experiments.
We help you get the best results
- Our technical support team will assist you throughout your projects with experimental design, sample preparation, troubleshooting, and service options.
- You can find a collection of FAQs and technical documents, including datasheets, protocols, and manuals.
- Training at Miltenyi University
- Protect your instrument with a comprehensive service contract.
autoMACS® Pro Separator
Benchtop instrument for fully automated cell separation.
Order no.: 130-092-545.
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