Removal of unwanted material from cell suspensions for more reliable genomic results

Working with samples with high cell viability and free of debris is a key prerequisite to obtain accurate and reliable results in single-cell sequencing. This is particularly important for gene expression analysis since only viable nucleated cells will generate functional libraries. Our strainers and removal reagents help you to clean up your single-cell suspensions from unwanted material such as, cell aggregates, dead cells, debris or erythrocytes to get the best possible genomic analysis results.

 

Genomic analysis of cell suspensions after cleanup

Clean up of tumor samples improves the quality of single-cell genomic analysis
Single-cell gene expression data quality was increased after tumor cell suspensions underwent cleanup steps

Clean up of tumor samples improves the quality of single-cell gene expression analysis

Application of different clean up steps on mouse tumor cell suspensions helps to remarkably increase the quality of single-cell genomic data.

Related PDFs

Dissociation of tumor tissue for single cell genomics (application note)
 

How to cleanup your cell suspensions prior to single-cell genomic analysis

Removal of large particles and cell aggregates

MACS SmartStrainers allow the removal or large particles and cell aggregates in cell suspensions from solid tissues or blood samples. Their special design enables a fast filtration without clogging and easily fit into 15 mL and 50 mL tubes.

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Not just cell strainers. SmartStrainers.

Removing large particles and cell aggregates in cell suspensions from solid tissues or blood samples provides purer samples that will produce higher quality data.

Required products

MACS SmartStrainers

MACS Handbook chapters

Sample preparation
Clean up of tumor samples improves the quality of single-cell genomic analysis
Increased cell viability rates of PBMC samples after removal of dead cells 

Dead cell removal

Use the Dead Cell Removal Kit for the fast and straight forward magnetic depletion of dead cells from blood samples, cryopreserved cells, or cell suspensions from fresh solid tissues containing robust cells, such as fibroblasts, epithelial cells, tumor cells, or immune cells. When combined with the autoMACS® Pro Separator, the process of dead cell removal can be performed in an automated way by just clicking a button.

Required products

Dead cell removal kit 
autoMACS Pro Separator 

Related PDFs

Increase the viability of your samples with the Dead Cell Removal Kit (application note)
Increased yield of viable adult mouse neural cells after debris removal and red blood cell lysis
Yield of viable adult mouse neural cells were increased by applying debris removal and red blood cell lysis

Debris removal and red blood cell lysis 

The Debris Removal Solution is a ready-to-use density gradient reagent that allows the fast and convenient removal of subcellular debris in cell suspensions containing fragile cells, such as adult neurons, adult cardiomyocytes or hepatocytes , and kidney. In addition, lysis of red blood cells with the Red Blood Cell Lysis Solution helps to increase the yields of viable nucleated cells.

Required products

Debris cell removal 
Red blood cell lysis 

Related PDFs

Automated adult and neonatal mouse brain dissociation and magnetic isolation of neurons increases efficiency and sensitivity for single-cell RNA sequencing and gene expression profiling (scientific poster)

Further information

Workflow overview

The complete workflow for cell and nuclei preparation for genomics
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Webinar

Preparation of cells and nuclei for genomic analysis
Product portfolio overview

Removal reagents

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