This application protocol describes step-by-step the immunostaining and clearing of mouse brain hemispheres using the MACS® Clearing Kit for downstream 3D imaging analysis. The MACS Clearing Kit enables the immunostaining and clearing of up to 20 brain hemispheres from mice of any age.
2.1.1 Fixation of neonatal mouse brain hemispheres (from mice of age ≤P7)
2.1.2 Fixation of adult mouse brain hemispheres (from mice of age >P7)
2.4.1 Dehydration of mouse brain hemispheres from ≤P7 mice
2.4.2 Dehydration of mouse brain hemispheres from >P7 mice
For more information about light sheet fluorescence microscopy refer to Light sheet microscopy.
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