Miltenyi Biotec-tested panel 18 (MBTP 18)
This application protocol describes the flow cytometric analysis of NK cells after spleen dissociation from healthy C57BL/6 mice. Mouse spleens are easily dissociated using the gentleMACS™ Dissociators to quickly obtain viable single cell suspensions ready for flow cytometric analysis.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) |
CD11b | REA592 | NK cell maturation | VioBlue® | 450/50 (violet) |
Viobility™ 405/520 Fixable Dye | - | Viability | - | 525/50 (violet) |
CD335 (NKp46) | REA815 | NK cells | FITC | 525/50 (blue) |
KLRG1 | REA1016 | Mature NK cells | PE | 585/40 (blue) |
CD3e | REA606 | Cell type exclusion | PE-Vio® 615 | 655-730 (blue) |
CD19 | REA749 | Cell type exclusion | PE-Vio 615 | 655-730 (blue) |
NK1.1 | PK136* | NK cells | PE-Vio 770 | 750LP (blue) |
CD27 | REA499 | NK cell maturation | APC | 655-730 (red) |
CD45 | REA737 | Leukocytes | APC-Vio 770 | 750LP (red) |
*alternative REAfinity™ Antibody available: NK1.1 Antibody, anti-mouse, PE-Vio 770, REAfinity
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