Miltenyi Biotec-tested panel 29 (MBTP 29)
This application protocol describes the flow cytometric analysis of antigen-specific CD4+ T cells from human PBMCs.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|Viobility™ 405/520 Fixable Dye||-||Viability||-||525/50 (violet)|
|CD3||BW264/56*||T cells||VioBlue®||450/50 (violet)|
|CD4||M-T466*||T helper cells||PerCP-Vio® 770||655–730 (blue)|
|CD154||REA238||Activation marker||PE||585/40 (blue)|
|CD185 (CXCR5)||REA103||Antigen-specific cells||PE-Vio 770||750 LP (blue)|
|CD183 (CXCR3)||REA232||Follicular T helper cells 1||Vio Bright FITC||525/50 (blue)|
|CD196 (CCR6)||REA190||Follicular T helper cells 17||APC||655-730 (red)|
*alternative REAfinity™ Antibody available
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