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Miltenyi Biotec-tested panel 33 (MBTP 33)
This application protocol describes the characterization of activated T cells within antigen-stimulated peripheral blood mononuclear cells (PBMCs) via simultaneous measurement of surface markers and intracellular cytokines using flow cytometry. Prior to antibody staining PBMCs were stimulated with a cocktail of PepTivator CMV pp65, PepTivator EBV consensus, and PepTivator AdV5 Hexon from Miltenyi Biotec.
|Specificity||Clone||Purpose||Fluorochrome||Detection filter (nm) (laser)|
|Viobility™ 405/452 Fixable Dye||-||Viability||-||450/50 (violet)|
|IFN-γ||REA600||T cell activation||Vio® R720||725/40 (red)|
|CD3||REA613||T cells||APC-Vio 770||785/62 (red)|
|CD4||REA623||T helper cells||Vio Bright B515||525/50 (blue)|
|CD8||REA734||Cytotoxic T cells||VioGreen™||525/50 (violet)|
|TNF-α||REA656||T cell activation||PE||579/34 (blue)|
|CD154||REA238||T cell activation||APC||667/30 (red)|
|CD14||REA599||Cell type exclusion||VioBlue®||450/50 (violet)|
|CD20||REA780||Cell type exclusion||VioBlue||450/50 (violet)|
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