Miltenyi Biotec-tested panel 30 (MBTP 30)
This application protocol describes the analysis of the human KIR repertoire by flow cytometry. An antibody panel is presented that allows the simultaneous assessment of the four major inhibitory KIRs (KIR2DL1, KIR2DL2/DL3/DS2, KIR3DL1) and an additional inhibitory NK cell receptor, namely NKG2A.
Table 1: Antibody panel.
Specificity | Clone | Purpose | Fluorochrome | Detection filter (nm) (laser) | |
Backbone staining cocktail | 7-AAD Staining Solution | - | Viability | - | 655-730 (blue) |
CD3 | REA613 | T cells | PerCP-Vio® 770 | 655-730 (blue) | |
CD14 | REA599 | Monocytes | PerCP-Vio 770 | 655-730 (blue) | |
CD45 | REA747 | Leukocytes | VioBlue® | 450/50 (violet) | |
CD56 | REA196 | NK cells | Vio Bright B515 | 525/50 (blue) | |
Single inhibitory receptor staining cocktail | CD158a (KIR2DL1) | REA284 | KIR2DL1+ NK cells | PE/APC* | 585/40 (blue)/ 655-730 (red) |
CD158b (KIR2DL2/DL3) | DX27** | KIR2DL2/DL3+ NK cells | PE/APC* | 585/40 (blue)/ 655-730 (red) | |
CD158e (KIR3DL1) | DX9** | KIR2DL1+ NK cells | PE/APC* | 585/40 (blue)/ 655-730 (red) | |
CD159a (NKG2A) | REA110 | NKG2A+ NK cells | PE/APC* | 585/40 (blue)/ 655-730 (red) |
Table 2: Complementary antibody cocktails for single inhibitory receptor stainings.
Inhibitory receptor of interest | CD158a (KIR2DL1) | CD158b (KIR2DL2/DL3) | CD158e (KIR3DL1) | CD159a (NKG2A) |
Antibody cocktail for single inhibitory receptor staining | CD158a (KIR2DL1)-PE | CD158b (KIR2DL2/DL3)-PE | CD158e (KIR3DL1)-PE | CD159a (NKG2A)-PE |
- | CD158a (KIR2DL1)-APC | CD158a (KIR2DL1)-APC | CD158a (KIR2DL1)-APC | |
CD158b (KIR2DL2/DL3)-APC | - | CD158b (KIR2DL2/DL3)-APC | CD158b (KIR2DL2/DL3)-APC | |
CD158e (KIR3DL1)-APC | CD158e (KIR3DL1)-APC | - | CD158e (KIR3DL1)-APC | |
CD159a (NKG2A)-APC | CD159a (NKG2A)-APC | CD159a (NKG2A)-APC | - |
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use.
If not otherwise specified, all marks displayed on the Miltenyi Biotec Internet site and documents are subject to the trademark rights of Miltenyi Biotec, including each of Miltenyi Biotec's primary brands, its model name plates, and its corporate logos and emblems.
For copyright note and a complete list of trademarks visit www.miltenyibiotec.com/legalnotes.
Seems like you are coming from USA!
Do you want to visit our website in your country?