The SARS-CoV-2 B Cell MicroBead Kit, human has been developed for the easy enrichment of SARS-CoV-2–specific B cells by magnetic separation, by binding of the SARS-CoV-2 specific proteins to the respective antigen-specific B cell receptor (BCR) on B cells circulating in the peripheral blood of individuals who developed a B cell response against SARS-CoV-2 proteins.

Specifications for SARS-CoV-2 B Cell MicroBead Kits, human

Overview

The SARS-CoV-2 B Cell MicroBead Kit, human has been developed for the easy enrichment of SARS-CoV-2–specific B cells by magnetic separation, by binding of the SARS-CoV-2 specific proteins to the respective antigen-specific B cell receptor (BCR) on B cells circulating in the peripheral blood of individuals who developed a B cell response against SARS-CoV-2 proteins.

Detailed product information

Background information

B cells, also known as B lymphocytes express, dependent on their maturation stage, the antigen-specific B cell receptor (BCR) on their surface or secrete antigen-specific antibodies. They are part of the adaptive immune system and are crucial to mount a humoral, long lasting sterile immunity. B cells also play a role as professional antigen-presenting cells and secrete cytokines. They circulate through the body via the peripheral blood and account for 2–10% of all lymphocytes. Mature B cells in blood express the pan B cell marker CD19 and most mature B cells except plasma cells express CD20 and CD22. Additionally, different subsets of memory B cells and plasma cells can be identified based on their expression of Ig isotypes (IgM, IgD, IgG, IgA, IgE)1. Antigen-specific B cells usually occur at a frequency less than 0.05% for a specific antigen, but their number can vary depending on the phase and type of antigen or immunization
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The quantitative and qualitative analysis of antigen-specific B cells specifically recognizing and reacting towards a defined antigen provide important information to understand their function in various immunological situations. The presence of these cells indicate that an individual is mounting an adaptive response to the specific, infective pathogen or to an immunization containing that specific antigen.⁵,⁶ Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces an adaptive immune response leading to SARS-CoV-2–specific immunoglobulin and SARS-CoV-2–specific memory B and memory T cells with variable persistency and antiviral efficacy.
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Analysis and enrichment of antigen-specific B cells can contribute to the understanding of the role of cellular and humoral response and thus to the protection from SARS-CoV-2 infection after a previous infection or immunization.
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Applications

Identification and characterization of SARS-CoV-2–specific B cells circulating in in peripheral blood of individuals who developed a B cell response against SARS-CoV-2 proteins:
  • Sensitive and specific quantification of the numbers of antigen specific B cells
  • Phenotyping of the maturation stage and isotype (IgG, IgA, IgM) of SARS-CoV-2–specific B cells
  • Flow sorting of SARS-CoV-2–specific B cells

Resources for SARS-CoV-2 B Cell MicroBead Kits, human

References for SARS-CoV-2 B Cell MicroBead Kits, human

Publications

  1. Perez-Andres, M. et al. (2010) Human peripheral blood B-cell compartments: a crossroad in B-cell traffic Cytometry B Clin Cytom. 78(1): 47-60
  2. Smith, M. J. et al. (2017) Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function J. Vis. Exp. 120: 55382
  3. Leyendeckers, H. et al. (1999) Correlation analysis between frequencies of circulating antigen-specific IgG-bearing memory B cells and serum titers of antigen-specific IgG Eur. J. Immunol. 29(4): 1406-1417
  4. Ward, S. M. et al. (2008) Direct ex vivo evaluation of long-lived protective antiviral memory B cell responses against hepatitis B virus J. Infect. Dis. 198(6): 813-817
  5. Hartley, G. E. et al. (2020) Rapid generation of durable B cell memory to SARS-CoV-2 spike and nucleocapsid proteins in COVID-19 and convalescence Sci Immunol 5(54): eabf8891
  6. Gaebler, C. et al. (2021) Evolution of antibody immunity to SARS-CoV-2 Nature 591: 639-644
  7. Dan, J. et al. (2021) Immunological memory to SARS-CoV-2 assessed for up to 8 months after infection. Science 371: eabf4063
  8. Sakharkar, M. et al. (2021) Prolonged evolution of the human B cell response to SARS-CoV-2 infection Sci Immunol 6(56): eabg6916
  9. Wang, Z. et al. (2021) mRNA vaccine-elicited antibodies to SARS-CoV-2 and circulating variants bioRxiv - the preprint server for Biology : 2021.01.15.426911
  10. Mazzoni, A. et al. (2021) First dose mRNA vaccination is sufficient to reactivate immunological memory to SARS-CoV-2 in ex COVID-19 subjects medRxiv - the preprint server for health sciences (doi.org/10.1101/2021.03.05.21252590)

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