Clone:
REA610
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
MHC class II IAk, Aßk, A beta K, Aalpha, H-2Aa, H2Aa, I-Aalpha, IAalpha

Specifications for MHC Class II (I-Ak) Antibody, anti-mouse, REAfinity™

Overview

Clone REA610 recognizes the β chain of the mouse MHC class II alloantigen I-Ak of H-2k bearing mouse strains. It also cross-reacts with the H-2f, H-2r, and H-2s haplotypes, but reactivity to other haplotypes (i.e., b, d, p, and q) has not been observed. It also cross-reacts with splenocytes from nonobese diabetic (NOD) mice, and with lymph node cells of BN, DA, WRA, and WRC rats. MHC class II is expressed on antigen-presenting cells, such as dendritic cells, monocytes, macrophages, B cells in lymphoid and non-lymphoid tissue, thymic epithelial cells, and on subsets of hematopoietic progenitor cells in the bone marrow.
Additional information: Clone REA610 displays negligible binding to Fc receptors.

Alternative names

MHC class II IAk, Aßk, A beta K, Aalpha, H-2Aa, H2Aa, I-Aalpha, IAalpha

Detailed product information

Technical specifications

CloneREA610
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenMHC Class II (I-Ak)
Alternative names of antigenMHC class II IAk, Aßk, A beta K, Aalpha, H-2Aa, H2Aa, I-Aalpha, IAalpha
Molecular mass of antigen [kDa]26
Distribution of antigenB cells, dendritic cells, macrophages, monocytes, thymic epithelial cells, hematopoietic stem and progenitor cells
Entrez Gene ID14960
RRIDAB_2652871, AB_2652872, AB_2652873, AB_2652874, AB_2652875, AB_2652876, AB_2652877, AB_2652878, AB_2652879, AB_2652880, AB_2652881, AB_2652870

References for MHC Class II (I-Ak) Antibody, anti-mouse, REAfinity™

Publications

  1. Bhattacharya, A. et al. (1981) A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J. Immunol. 127(6): 2488-2495
  2. Nelson, C. A. et al. (1996) A negatively charged anchor residue promotes high affinity binding to the MHC class II molecule I-Ak. J. Immunol. 157(2): 755-762
  3. Ting, J. P. et al. (2002) Genetic control of MHC class II expression. Cell 109: 21-33

Related products for
MHC Class II (I-Ak) Antibody, anti-mouse, REAfinity™

2 products available