REAlease® Anti-Siglec-F Complexes, mouse

REAlease
®
Anti-Siglec-F Complexes
, mouse

Clone: REAL291 | Dilution: 1:50
Clone REAL291 is an antibody fragment derived from the full Anti-Siglec-F antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.
Clone REAL291 recognizes the mouse siglec-F antigen, also kown as siglec-5, which belongs to the sialic acid-binding immunoglobulin-type lectins (siglec) family. Siglecs are expressed on immune cells and are a subset of the I-type lectins. Siglec-F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. In mouse, siglec-F is an inhibitory receptor on eosinophils.
The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

SIGLECF, SIGLEC5, mSiglec-F, Siglec-5

Technical specifications

  • Antigen: Siglec-F
  • Clone: REAL291
  • Isotype control: REAL Control
  • Alternative names of antigen: SIGLECF, SIGLEC5, mSiglec-F, Siglec-5
  • Distribution of antigen: bone marrow, granulocytes, neutrophils, monocytes, eosinophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: APC, PE
  • Selected references

    1. Tateno, H. et al. (2005) Mouse Siglec-F and human Siglec-8 are functionally convergent paralogs that are selectively expressed on eosinophils and recognize 6-sulfo-sialyl Lewis X as a preferred glycan ligand. Glycobiology 15(11): 1125-1135
    2. Zhang, M. et al. (2007)
      Defining the
      in vivo
      function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.
      Blood 109(10): 4280-4287
    3. Guo, J. P. et al. (2011) Characterization of expression of glycan ligands for Siglec-F in normal mouse lungs. Am. J. Respir. Cell Mol. Biol. 44(2): 238-243
Product options: 4
30 µg in 200 µL (1)
EUR 80.00
30 µg in 200 µL (1)
EUR 80.00
for 100
tests
EUR 125.00
for 100
tests
EUR 125.00

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