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Human peripheral blood cells after erythrocyte lysis were stained with CD312 (EMR2) antibodies or with the corresponding REA Control (S) antibodies (left images) as well as with CD14 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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