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Human peripheral blood mononuclear cells (PBMCs) were spiked with human umbilical vein endothelial cells (HUVECs), stained with CD309 (VEGFR-2) antibodies as well as with CD45 antibodies, and analyzed by flow cytometry using the MACSQuant®
Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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