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Mouse CD255 transfected L5178Y cells (right peak) and untransfected cells were stained with CD255 (TWEAK) antibodies conjugated to PE (A), APC (B) and Biotin (C) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cells labeled with Biotin were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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