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EA-hy 926 cells were serum starved overnight, resuspended in serum-free DMEM, and rested for 20 minutes at 37 °C, and then either left unstimulated (left peak) or stimulated with 1 mM pervanadate for 20 minutes at 37 °C. Cells were fixed and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with CD202b pY1102 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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