CD16b antibodies, human

CD16b antibodies, human

Clone: REA589 | Dilution: 1:50
Clone REA589 recognizes the human CD16b antigen, a glycosylphosphatidylinositol-anchored protein known as low affinity immunoglobulin gamma Fc region receptor III-B. CD16 is expressed in two different isoforms, FcγRIIIa (CD16a), which is found on human NK cells, macrophages, and mast cells, and FcγRIIIb (CD16b) Fc receptors, that bind to the Fc portion of IgG antibodies which mediates NK cell activating. CD16b is polymorphic and has two codominant alleles that are related to Neutrophil Antigen 1 (NA1) and Neutrophil Antigen 2 (NA2). Clone REA589 is present on polymorphonuclear leukocytes (neutrophils) expressing the NA1 molecule.
Additional information: Clone REA589 displays negligible binding to Fc receptors.

Alternative names

FCGR3B, Fc-gamma RIIIb, FCG3, FCGR3, FcR-10, FcRIII, FcRIIIb

Technical specifications

  • Antigen: CD16b
  • Clone: REA589
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: FCGR3B, Fc-gamma RIIIb, FCG3, FCGR3, FcR-10, FcRIII, FcRIIIb
  • Entrez Gene ID: 2215
  • Molecular mass of antigen [kDa]: 21
  • Distribution of antigen: neutrophils
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE
  • Selected references

    1. Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
    2. Peltz, G. A. et al. (1989) Human Fc gamma RIII: cloning, expression, and identification of the chromosomal locus of two Fc receptors for IgG. Proc. Natl. Acad. Sci. U.S.A. 86(3): 1013-1017
    3. Gessner, J. E. et al. (1995) The human low affinity immunoglobulin G Fc receptor III-A and III-B genes. Molecular characterization of the promoter regions. J. Biol. Chem. 270(3): 1350-1361
    4. Monti, M. et al. (2017) Integrin-dependent cell adhesion to neutrophil extracellular traps through engagement of fibronectin in neutrophil-like cells. PLoS One 12
Product options: 2
130-123-643

CD16b-PE, human

for 30
tests
(1)
EUR 90.00
130-123-557

CD16b-PE, human

for 100
tests
(1)
EUR 220.00

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