CD16 antibodies, human

CD16 antibodies, human

Clone: REA423
Clone REA423 recognizes the human CD16 antigen, a single-pass type I membrane protein which is also known as low affinity immunoglobulin gamma Fc region receptor III (FcRIII). CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils. The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone REA423 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes.
Additional information: Clone REA423 displays negligible binding to Fc receptors.

Alternative names

FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII

Technical specifications

  • Antigen: CD16
  • Clone: REA423
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control (S) antibodies
  • Alternative names of antigen: FCGR3A, FCGR3B, FcR-10, IGFR3, IMD20, FcRIII
  • Entrez Gene ID: 2214, 2215
  • Molecular mass of antigen [kDa]: 48
  • Cross-reactivity:
    rhesus monkey (
    Macaca mulatta
    )
    ,
    cynomolgus monkey (
    Macaca fascicularis
    )
    , capuchin monkey,
    chimpanzee (
    Pan troglodytes
    )
    ,
    common marmoset (
    Callithrix jacchus
    )
    ,
    cotton-top tamarin (
    Saguinus oedipus
    )
    ,
    olive baboon (
    Papio anubis
    )
    ,
    pigtail monkey (
    Macaca nemestrina
    )
    ,
    squirrel monkey (
    Saimiri sciureus
    )
  • Distribution of antigen: NK cells, monocytes
  • Product format: Antibodies are supplied in phosphate-buffered saline (PBS), pH 7.2. The product contains no preservative and is sterile filtered. Always handle under aseptic conditions.
  • Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: pure
  • Selected references

    1. Croci, S. et al. (2018) Higher frequencies of lymphocytes expressing the natural killer group 2D receptor in patients with Behçet disease. Front Immunol 9: 2157
    2. Veluchamy, J. et al. (2017)
      In vivo
      efficacy of umbilical cord blood stem cell-derived NK cells in the treatment of metastatic colorectal cancer.
      Front Immunol 8: 87
    3. Ravetch, J. V. et al. (1989) Alternative membrane forms of Fc gamma RIII(CD16) on human natural killer cells and neutrophils. Cell type-specific expression of two genes that differ in single nucleotide substitutions. J. Exp. Med. 170(2): 481-497
    4. Ziegler-Heitbrock, L. (2007)
      The CD14
      +
      CD16
      +
      blood monocytes: their role in infection and inflammation.
      J. Leukoc. Biol. 584(592): 584-592
    5. Romee, R. et al. (2013) NK cell CD16 surface expression and function is regulated by a disintegrin and metalloprotease-17 (ADAM17). Blood 121(18): 3599-3608
  • Brochures and posters

Product options: 1
130-108-027

CD16 pure, human

100 µg in 100 µL
EUR 151.00