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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Human peripheral blood mononuclear cells (PBMCs) were either left unstimulated (left peak) or stimulated with CD3/CD28 antibodies for 15 minutes on ice followed by stimulation with goat anti-mouse IgG for 15 minutes on ice, and then allowed to undergo phosphorylation at 37 °C for 5 minutes. Cells were fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-ZAP70 pY292 antibodies. Flow cytometry was performed with the MACSQuant®
cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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