Anti-Sca-1 antibodies, mouse

Anti-Sca-1 antibodies, mouse

Clone: REA422 | Dilution: 1:50
Clone REA422 recognizes the mouse stem cell antigen-1 (SCA-1) antigen, a 18 kDa glycosylphosphatidylinositol (GPI) linked protein which is also known as lymphocyte antigen 6A and 6E (Ly-6A/E). Clone REA422 specifically recognizes both Ly-6E.1 and Ly-6A.2, which are gene products of the two Ly-6A/E alleles expressed in different mouse strains (Ly-6E.1: e.g. BALB/c, C3H, NZB; Ly-6A.2: e.g. C57BL/6, SJL, 129, AKR). Sca-1 is expressed on hematopoietic stem cells and progenitor cells in mouse bone marrow and is one of the defining markers for so-called KSL (c-kit
+
Sca-1
+
Lin
-
) cells. Sca-1 in combination with CD105 (Endoglin) expression has been used to define long-term repopulating hematopoietic stem cells (LTR-HSCs) in mouse bone marrow. In addition, Sca-1
+
bone marrow cells also contain the mesenchymal stem cell fraction. Sca-1
+
cells have been shown to give rise to hepatocytes
in vivo
and neural cells
in vitro
. Furthermore, Sca-1 is expressed on stem cells in a variety of nonhematopoietic tissues, such as adult liver, heart, and prostate.
Additional information: Clone REA422 displays negligible binding to Fc receptors.

Alternative names

LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP

Technical specifications

  • Antigen: Sca-1
  • Clone: REA422
  • Isotype: recombinant human IgG1
  • Isotype control: REA Control antibodies
  • Alternative names of antigen: LY6A, Ly-6A.2, Ly-6A/E, Ly-6E.1, TAP
  • Entrez Gene ID: 110454
  • Molecular mass of antigen [kDa]: 9
  • Distribution of antigen: hematopoietic stem cells
  • Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
  • Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
  • Storage: Store protected from light at 2–8 °C. Do not freeze.
  • Available conjugates: PE, APC, VioBright FITC
  • Selected references

    1. Bradfute, S. B. et al. (2005) Roles of Sca-1 in hematopoietic stem/progenitor cell function. Exp. Hematol. 33(7): 836-843
    2. Khan, K. D. et al. (1990) Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells. Mol. Cell. Biol. 10(10): 5150-5159
    3. Houlihan, D. D. et al. (2012) Isolation of mouse mesenchymal stem cells on the basis of expression of Sca-1 and PDGFR-α. Nat. Protoc. 7(12): 2103-2111
  • Customer reports and application notes

Product options: 4
30 µg in 200 µL (1)
EUR 85.00
30 µg in 200 µL (1)
EUR 65.00
150 µg in 1 mL (1)
EUR 190.00
30 µg in 200 µL (1)
EUR 65.00

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