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Miltenyi Biotec distribution:
As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
StraightFrom® Leukopak® MicroBeads allow for the magnetic cell separation of large leukocyte numbers directly from Leukopak® (StemExpress, LCC). Leukocyte subsets such as CD4+, CD8+, CD14+, or CD19+ cells can be purified using either semi-or fully automated using MultiMACS™ Instruments. Pre-enrichment of white blood cells, e.g. by density gradient centrifugation, is not required. The purified cells are well-suited for further flow cytometric analyses, molecular biology applications, and functional studies.
Separation of a Leukopak® sample using the StraightFrom Leukopak® CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD14-APC, CD15-PE-Vio® 770, as well as CD45-VioBlue® and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.