Memory CD4+ T Cell Isolation Kit, human

Memory CD4
T Cell Isolation Kit
, human

The Memory CD4
T Cell Isolation Kit was developed for the isolation of untouched memory T helper cells from human peripheral blood.

Detailed separation procedure

For magnetic depletion of naive T cells, CD8
T cells, B cells, NK cells, γ/δ T cells, monocytes, DCs, granulocytes, platelets, and erythroid cells, PBMCs are incubated with a cocktail of biotinylated CD45RA, CD8, CD14, CD16, CD19, CD56, CD36, CD123, anti-TCRγ/δ, and CD235a (glycophorin A) antibodies. These cells are subsequently magnetically labeled with Anti-Biotin MicroBeads for depletion. The Memory CD4
T Cell Isolation Kit provides a fast and efficient way to isolate untouched memory CD4
T cells.

Downstream applications

memory T cells isolated with the Memory CD4
T Cell Isolation Kit are used when direct labeling of cell surface molecules could interfere with downstream applications, e.g., in studies on memory T helper cell activation. Untouched memory CD4
T cells can also be used for further isolation of specific subsets, such as CCR8


MS, LS, or autoMACS
  • Selected references

    1. Soler, D. et al. (2006) CCR8 expression identifies CD4 memory T cells enriched for FOXP3+ regulatory and Th2 effector lymphocytes. J. Immunol. 177: 6940-6951
Product options: 1
for 1×
total cells
EUR 802.00

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