Human IL-15

Human IL-15

Recombinant human IL-15 (interleukin 15) promotes proliferation of activated T, NK, and B cells . The regulatory cytokine IL-15 has important diverse roles in the immune system. It is structurally related to IL-2 and they share many functional characteristics. The recombinant protein is optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Human IL-15 can be used for a variety of applications, including:
  • Activation and expansion of NK and NKT cells.
  • In vitro differentiation of NK cells, e.g., from purified CD34+ cells.
  • In vitro T cell expansion, e.g., of naive CD8+ T cells, and T cell activation, e.g., of CTLs.

Background information

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. IL-15 is a potent lymphoid cell growth factor. It stimulates the proliferation of activated T cells and promotes the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, proliferation, and activation of NK cells as well as B cell growth and immunoglobulin production. In addition, IL-15 is important for the maintenance of CD8
+
memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Quality description

Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

  • Proliferation of CTLL-2 cells (NIBSC 95/554)
  • premium grade: ≥ 5×
    10
    6
    U/mg
    (typical activity: ≥ 1.1×
    10
    7
    U/mg
    )
  • research grade: ≥ 2×
    10
    6
    U/mg
  • Selected references

    1. Gordy, L. E. et al. (2011) IL-15 regulates homeostasis and terminal maturation of NKT cells. J. Immunol. 187(12): 6335-6345
    2. Juelke, K. et al. (2010)
      CD62L expression identifies a unique subset of polyfunctional CD56
      dim
      NK cells.
      Blood 116(8): 1299-1307
    3. Alvarez-Breckenridge, C. A. et al. (2012) The histone deacetylase inhibitor valproic acid lessens NK cell action against oncolytic virus-infected glioblastoma cells by inhibition of STAT5/T-BET signaling and generation of gamma interferon. J. Virol. 86(8): 4566-4577
    4. Fuchs, S. et al. (2014)
      Patients with T
      +/low
      NK
      +
      IL-2 receptor γ chain deficiency have differentially-impaired cytokine signaling resulting in severe combined immunodeficiency.
      Eur. J. Immunol. 44(10): 3129-3140
    5. Dietz, L. et al. (2010) Tracking human contact allergens: from mass spectrometric identification of peptide-bound reactive small chemicals to chemical-specific naive human T-cell priming. Toxicol. Sci. 117(2): 336-347
  • Certificates

    Please follow this
    link
    to search for Certificates of Analysis (CoA) by lot number.
Product options: 6
E. coli
10 µg
EUR 153.00
E. coli
25 µg
EUR 287.00
E. coli
10 µg
EUR 189.00
E. coli
25 µg
EUR 359.00
E. coli
100 µg
EUR 620.00
E. coli
1000 µg
EUR 2,752.00

Related products

1 products available