Working with antigen-specific T cells in Immuno-Oncology

Analysis of antigen-specific T cells is essential for the understanding of fundamental immunological processes in the context of immuno-oncology (IO), infectious diseases, autoimmunity as well as immune tolerance.  
In immuno-oncology they facilitate discovery of neo-antigens, but are also viewed as important immunotherapy effectors. In this context, their ability, to recognize and kill cancer cells make them an ideal target for IO research.   

However,  working with antigen-specific T cells poses several challenges to researchers. Especially their low frequencies as rare cell population often hampers a reliable analysis. Specificity, inflammatory environment, subtype as well as detection limit and background are determining factors and thus require optimized procedures in order to facilitate a successful experiment. 
 

Miltenyi Biotec offers a complete research workflow to enable rare cell analysis of antigen-specific T cells: 

• Antigen-specific stimulation with PepTivator Peptide Pools 
• Enrichment based on specific activation marker expression of CD137 and CD154
• Flow analysis with minimal background using REAfinity Recombinant Antibodies
• Convenient and reproducible cytokine analysis with Flow Cytometry Kits 
• Rare cell GMP-compliant flow sorting using the MACSQuant Tyto Cell Sorter
   

Highlight: GMP-compliant cell sorting of antigen-specific T cells in clinical settings