The B Cell Expansion Kit has been developed for the activation and expansion of human B cells. The CD40-Ligand Multimer mimics a T cell-dependent activation of B cells. The B cell expansion is achieved by culturing and restimulation at day 7 and 10 of culture.

Data and images for B Cell Expansion Kit, human

Figures

Figure 1

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The components of the CD40-Ligand Multimer Kit were pre-incubated 30 minutes at room temperature. B cells were isolated using CD19 MicroBeads and cultured (StemMACS HSC Expansion Media XF supplemented with 5% AB serum and 2 µL IL-4) at a final density or 0.15×10
6
cells per mL (low density).
On day 7 and 10 cells were harvested, counted, and restimulated. All cells were harvested, counted and analyzed on day 14. With the low density protocol a B cell expansion of up to 10-fold can be achieved.

Figure 1

The components of the CD40-Ligand Multimer Kit were pre-incubated 30 minutes at room temperature. B cells were isolated using CD19 MicroBeads and cultured (StemMACS HSC Expansion Media XF supplemented with 5% AB serum and 2 µL IL-4) at a final density or 0.15×10
6
cells per mL (low density).
On day 7 and 10 cells were harvested, counted, and restimulated. All cells were harvested, counted and analyzed on day 14. With the low density protocol a B cell expansion of up to 10-fold can be achieved.

Specifications for B Cell Expansion Kit, human

Overview

The B Cell Expansion Kit has been developed for the activation and expansion of human B cells. The CD40-Ligand Multimer mimics a T cell-dependent activation of B cells. The B cell expansion is achieved by culturing and restimulation at day 7 and 10 of culture.

Detailed product information

Background information

CD40-Ligand, also known as CD40L, CD154, TRAP, or TNFSF5 is a member of the TNF superfamily transiently expressed on the surface of activated CD4
+
T lymphocytes. It is either expressed membrane-bound or proteolytically released as a soluble form which comprises 2/3 of the extracellular domain. Its receptor CD40 is found on antigen-presenting cells such as B cells, dendritic cells, and macrophages. CD40L-CD40 interaction is important in T cell-APC (antigen-presenting cell) interaction and is e.g. involved in B cell differentiation and proliferation, isotype class-switching, and protection of B cells from apoptosis. The B Cell Expansion Kit contains the CD40-Ligand and a cross-linking antibody for multimerization to increase the biological activity of CD40-Ligand, as well as premium grade Human IL-4 and StemMACS HSC Expansion Medium XF, human.

Applications

Activation and expansion of human B cells.

Resources for B Cell Expansion Kit, human

Certificates

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References for B Cell Expansion Kit, human

Publications

  1. Johnson, M. J. et al. (2018) Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism. Sci Rep 8(1): 12144
  2. Koliha, N. et al. (2016) Melanoma affects the composition of blood cell-derived extracellular vesicles. Front Immunol 7: 282

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