The Viobility™ Fixable Dyes allow the discrimination between live and apoptotic or dead cells by flow cytometry. The dyes are suitable for both fixed and unfixed cells.
Following reagents are available, addressing different fluorescent channels:
  • Viobility 405/452 Fixable Dye (Ex.: 405 nm, Em.: 452 nm)
  • Viobility 405/520 Fixable Dye (Ex.: 405 nm, Em.: 520 nm)
  • Viobility 488/520 Fixable Dye (Ex.: 488 nm, Em.: 520 nm)

Data and images for Viobility™ Fixable Dyes

Figures

Figure 1

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Cells stained with propidium iodide (A) or Viobility Fixable Dye (B). Cells in light orange represent apoptotic and dead cells.

Figure 1

Cells stained with propidium iodide (A) or Viobility Fixable Dye (B). Cells in light orange represent apoptotic and dead cells.

Figure 2

A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs) were stained with propidium iodide (A) or propidium iodide and Viobility 488/520 Fixable Dye (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
A):
B):
View details

Figure 2

A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs) were stained with propidium iodide (A) or propidium iodide and Viobility 488/520 Fixable Dye (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer.
View details

Figure 2

A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs) were stained with propidium iodide (A) or propidium iodide and Viobility 488/520 Fixable Dye (B) and analyzed by flow cytometry using the MACSQuant
®
Analyzer.

Figure 3

A) A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs), either unstained (black line) or stained with Viobility 488/520 Fixable Dye (purple line), were analyzed by flow cytometry using the MACSQuant Analyzer.
B) Two samples of human PBMCs (treated as above) were stained with Viobility 405/452 Fixable Dye. One sample was fixed, using the Inside Stain Kit (purple line), the other sample was not fixed (black line). Cells were analyzed by flow cytometry using the MACSQuant Analyzer.
A):
B):
View details

Figure 3

A) A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs), either unstained (black line) or stained with Viobility 488/520 Fixable Dye (purple line), were analyzed by flow cytometry using the MACSQuant Analyzer.
B) Two samples of human PBMCs (treated as above) were stained with Viobility 405/452 Fixable Dye. One sample was fixed, using the Inside Stain Kit (purple line), the other sample was not fixed (black line). Cells were analyzed by flow cytometry using the MACSQuant Analyzer.
View details

Figure 3

A) A mixture of live and heat-treated (for 10 minutes at 95 °C) human peripheral blood mononuclear cells (PBMCs), either unstained (black line) or stained with Viobility 488/520 Fixable Dye (purple line), were analyzed by flow cytometry using the MACSQuant Analyzer.
B) Two samples of human PBMCs (treated as above) were stained with Viobility 405/452 Fixable Dye. One sample was fixed, using the Inside Stain Kit (purple line), the other sample was not fixed (black line). Cells were analyzed by flow cytometry using the MACSQuant Analyzer.

Figure 4

Jurkat cells were treated with 1 µM of Apoptosis-inducing Staurosporin prior to staining with Viobility 488/520 Fixable Dye. Figure A shows untreated cells, Figure B shows Staurosporin-treated cells.
A):
B):
View details

Figure 4

Jurkat cells were treated with 1 µM of Apoptosis-inducing Staurosporin prior to staining with Viobility 488/520 Fixable Dye. Figure A shows untreated cells, Figure B shows Staurosporin-treated cells.
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Figure 4

Jurkat cells were treated with 1 µM of Apoptosis-inducing Staurosporin prior to staining with Viobility 488/520 Fixable Dye. Figure A shows untreated cells, Figure B shows Staurosporin-treated cells.

Specifications for Viobility™ Fixable Dyes

Overview

The Viobility™ Fixable Dyes allow the discrimination between live and apoptotic or dead cells by flow cytometry. The dyes are suitable for both fixed and unfixed cells.
Following reagents are available, addressing different fluorescent channels:
  • Viobility 405/452 Fixable Dye (Ex.: 405 nm, Em.: 452 nm)
  • Viobility 405/520 Fixable Dye (Ex.: 405 nm, Em.: 520 nm)
  • Viobility 488/520 Fixable Dye (Ex.: 488 nm, Em.: 520 nm)

Detailed product information

The Viobility™ Fixable Dyes have been developed for the detection and discrimination of dead cells. These dyes react with the primary amine groups of proteins which can be found on the cell surface as well as intracellularly.
In contrast to viable cells, dead cells exhibit a compromised cell membrane allowing the Viobility Fixable Dyes to enter the cell and stain proteins within the cell. This results in a up to 50-fold brighter fluorescent staining and thus allows the discrimination between dead and viable cells by using a flow cytometer.
Viobility Fixable Dyes show certain advantages over traditional dead cell–staining dyes like propidium iodide (PI):
  • non-toxic to cells
  • less spillover into other fluorescent channels
  • more flexibility in antibody panel design, as dyes are available for various excitation and emission wavelengths

References for Viobility™ Fixable Dyes

Publications

  1. Scheffold, A: et al. (2018) CD137+CD154- Expression As a Regulatory T Cell (Treg)-Specific Activation Signature for Identification and Sorting of Stable Human Tregs from In Vitro Expansion Cultures. Front Immunol 9

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