Clone:
F38-2E2
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1κ
Applications:
FC
Alternative names:
HAVCR2, CD366, HAVcr-2, KIM-3, TIMD-3

Extended validation for TIM-3 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with F38-2E2
2321C-
7D3-
REA635++
REAL226++
Cells were incubated with an excess of purified unconjugated TIM-3 (F38-2E2) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD366 (TIM-3). Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 3 days were stained with CD366 (TIM-3) antibodies and with a suitable counterstaining. As a control, CD366 (TIM-3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD366 (TIM-3). Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 3 days were stained with CD366 (TIM-3) antibodies and with a suitable counterstaining. As a control, CD366 (TIM-3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD366 (TIM-3). Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 3 days were stained with CD366 (TIM-3) antibodies and with a suitable counterstaining. As a control, CD366 (TIM-3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD366 (TIM-3). Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 3 days were stained with CD366 (TIM-3) antibodies and with a suitable counterstaining. As a control, CD366 (TIM-3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD366 (TIM-3). Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 3 days were stained with CD366 (TIM-3) antibodies and with a suitable counterstaining. As a control, CD366 (TIM-3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD366 (TIM-3). Human peripheral blood mononuclear cells (PBMCs) stimulated with CD3/CD28 antibodies (T Cell TransAct™) for 3 days were stained with CD366 (TIM-3) antibodies and with a suitable counterstaining. As a control, CD366 (TIM-3) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TIM-3 (F38-2E2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TIM-3 (F38-2E2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TIM-3 (F38-2E2). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TIM-3 Antibody, anti-human

Overview

The monoclonal antibody F38-2E2 recognizes human cell surface molecule TIM-3, which is expressed on activated T cells, especially Tʜ1 cells, monocytes, macrophages, and dendritic cells. TIM-3 has been proposed to inhibit Tʜ1-mediated immune responses and regulates Tʜ1 and Tʜ17 cytokine secretion. The interaction of TIM-3 and its ligand galectin-9 induces apoptosis of Tʜ1 cells.

Alternative names

HAVCR2, CD366, HAVcr-2, KIM-3, TIMD-3

Detailed product information

Technical specifications

CloneF38-2E2
Clonalitymonoclonal
Isotypemouse IgG1κ
Isotype controlIsotype Control Antibody, mouse IgG1
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman
AntigenTIM-3
Alternative names of antigenHAVCR2, CD366, HAVcr-2, KIM-3, TIMD-3
Molecular mass of antigen [kDa]31
Entrez Gene ID84868
RRIDAB_2733706, AB_2752190, AB_2752171, AB_2654188, AB_2654189, AB_2733705

Resources for TIM-3 Antibody, anti-human

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for TIM-3 Antibody, anti-human

Publications

  1. Monney, L. et al. (2002) Tʜ1-specific cell surface protein Tim-3 regulates macrophage activation and severity of an autoimmune disease. Nature 415: 536-541
  2. Zhu, C. et al. (2005) The Tim-3 ligand galectin-9 negatively regulates T helper type 1 immunity. Nat. Immunol. 6(12): 1245-1252
  3. Hastings, W. D. et al. (2009)
    TIM-3 is expressed on activated human CD4
    +
    T cells and regulates Tʜ1 and Tʜ17 cytokines.
    Eur. J. Immunol. 39(9): 2492-2501
  4. Hirsch, S. et al. (1983) Polymorphic expression of a neutrophil differentiation antigen revealed by monoclonal antibody 7/4. Immunogenetics 18(3): 229-239

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