Clone:
REA536
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Vstm3, WUCAM

Extended validation for TIGIT Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA536
GIGD7++
1G9++
4D4/mTIGIT++
2190A (rabb IgG)++
826118 (rIgG1)++
Cells were incubated with an excess of purified unconjugated TIGIT (REA536) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using TIGIT (REA536). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using TIGIT (REA536). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using TIGIT (REA536). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for TIGIT Antibody, anti-mouse, REAfinity™

Overview

Clone REA536 recognizes the mouse T cell immunoreceptor with Ig and ITIM domains (TIGIT) antigen, a single-pass type I membrane protein which is also known as V-set and transmembrane domain-containing protein 3 (Vstm3). TIGIT belongs to a PVR family of type 1 proteins that also binds to CD155 and CD112 ligands. It is expressed on peripheral memory and regulatory CD4
+
T cells and NK cells and is upregulated following activation on naive CD4
+
T cells. Its interaction with CD155 induces tolerogenic dendritic cells that impaire T cell proliferation and inhibit IFN-γ production from responding T cells. Like the well-characterized CD28/CTLA-4 costimulatory pathway, a newly emerging pathway consisting of CD226 and TIGIT has been associated with susceptibility to multiple autoimmune diseases.
Additional information: Clone REA536 displays negligible binding to Fc receptors.

Alternative names

Vstm3, WUCAM

Detailed product information

Technical specifications

CloneREA536
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenTIGIT
Alternative names of antigenVstm3, WUCAM
Molecular mass of antigen [kDa]24
Distribution of antigenT cells, NK cells, thymocytes, T helper cells
Entrez Gene ID100043314
RRIDAB_2751782, AB_2801759

References for TIGIT Antibody, anti-mouse, REAfinity™

Publications

  1. Joller, N. et al. (2014) Treg cells expressing the coinhibitory molecule TIGIT selectively inhibit proinflammatory Tʜ1 and Tʜ17 cell responses. Immunity 40(4): 569-581
  2. Stanietsky, N. et al. (2013) Mouse TIGIT inhibits NK-cell cytotoxicity upon interaction with PVR. Eur. J. Immunol. 43(8): 2138-2150
  3. Pauken, K. E. et al. (2014) TIGIT and CD226: tipping the balance between costimulatory and coinhibitory molecules to augment the cancer immunotherapy toolkit. Cancer Cell 26(6): 785-787

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