The REAlease
®
CD8 MicroBead Kit has been developed for positive selection of CD8
+
T cells from PBMCs. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Data and images for
REAlease
®
CD8 MicroBead Kit
, human

Figures

Figure 1

View details
Step-by-step REAlease MicroBeads separation procedure

Figure 1

Step-by-step REAlease MicroBeads separation procedure

Figure 2

Example of a separation using the REAlease® CD8 MicroBead Kit
A) CD8
+
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8
+
cells"), whereas the label-free CD8
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).
A) Cell purity
Before separation
Label-free CD8
+
cells
View details

Figure 2

Example of a separation using the REAlease® CD8 MicroBead Kit
A) CD8
+
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8
+
cells"), whereas the label-free CD8
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).
View details

Figure 2

Example of a separation using the REAlease® CD8 MicroBead Kit
A) CD8
+
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8
+
cells"), whereas the label-free CD8
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).
B) Label-free cells: REAlease Biotin Complex release
Before separation
MicroBead-free CD8
+
cells
Label-free CD8
+
cells
View details

Figure 2

Example of a separation using the REAlease® CD8 MicroBead Kit
A) CD8
+
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8
+
cells"), whereas the label-free CD8
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).
View details

Figure 2

Example of a separation using the REAlease® CD8 MicroBead Kit
A) CD8
+
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8
+
cells"), whereas the label-free CD8
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).
View details

Figure 2

Example of a separation using the REAlease® CD8 MicroBead Kit
A) CD8
+
T cells were isolated from human PBMCs using the REAlease CD8 MicroBead Kit, MS Columns, and a MiniMACS™ Separator. Cells were fluorescently stained with CD8-PE and analyzed by flow cytometry using the MACSQuant
®
Analyzer X. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
B) The efficient removal of all labels was shown by using Anti-Biotin-APC to analyze the cells by flow cytometry for the presence of REAlease Biotin Complex. Directly after isolation, the cells showed staining of biotin ("MicroBead-free CD8
+
cells"), whereas the label-free CD8
+
cells after the REAlease Biotin Complex release were negative for biotin similar to the non-labeled cells before separation.
Release efficiency was higher than 99% for the REAlease Anti-Biotin MicroBeads (CD8).

Specifications for
REAlease
®
CD8 MicroBead Kit
, human

Overview

The REAlease
®
CD8 MicroBead Kit has been developed for positive selection of CD8
+
T cells from PBMCs. The kit contains an indirect magnetic labeling system for obtaining target cells free of MicroBeads and REAlease Biotin Complex.

Detailed product information

Background information

The CD8 antigen forms a complex together with the T cell receptor and acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8
+
cytotoxic T cells. The CD8 molecule consists of either an α/β heterodimer or an α/α homodimer. It is expressed strongly on cytotoxic T cells and dimly on a subset of NK cells. CD8 is found on most thymocytes and on about one third of all peripheral blood T cells. CD8
+
cytotoxic T cells play an important role in the killing of virus-infected cells and tumor cells.

Downstream applications

  • Isolation of specific CD8+ cell subsets. The selected CD8+ cells can be sorted according to a second marker of interest, e.g., CD45RA, CD45RO, CD16, CD251, CD272, CD28, CD30, CD56, CD62L, or CD69.
  • Isolation of CD8+ cell subset which needs to be label-free.

Resources for
REAlease
®
CD8 MicroBead Kit
, human

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®
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, human

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