Clone:
REA1162
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
NKR-P1 40, CD161c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C

Extended validation for NK1.1 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1162
PK136++
694370+
Cells were incubated with an excess of purified unconjugated NK1.1 (REA1162) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for NK1.1. Splenocytes derived from B57BL/6 mice were stained with NK1.1 antibodies and with a suitable counterstaining. As a control, NK1.1antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (REA1162). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (REA1162). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (REA1162). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (REA1162). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using NK1.1 (REA1162). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for NK1.1 Antibody, anti-mouse, REAfinity™

Overview

Clone REA1162 recognizes the mouse NK1.1 antigen, a single-pass type II membrane protein also known as CD161 or killer cell lectin-like receptor subfamily B member 1. NK1.1 is expressed by natural killer cells and a subset of T cells in the mouse strains C57BL/6, FVB/N, and NZB, but not in the mouse strains AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. It plays a stimulatory role on natural killer cells cytotoxicity. Activation by cross-linking of the receptor induces Ca
2+
mobilization and interferon-gamma production.
Additional information: Clone REA1162 displays negligible binding to Fc receptors.

Alternative names

NKR-P1 40, CD161c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C

Detailed product information

Technical specifications

CloneREA1162
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenNK1.1
Alternative names of antigenNKR-P1 40, CD161c, Ly-55c, NK1.1, NKR-P1.9, NKR-P1C
Molecular mass of antigen [kDa]25
Distribution of antigenNK cells, T cells, NKT cells
Entrez Gene ID17059
RRIDAB_2752122, AB_2752120, AB_2752123, AB_2752124, AB_2752125, AB_2752126, AB_2752127, AB_2752121, AB_2752128

Resources for NK1.1 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for NK1.1 Antibody, anti-mouse, REAfinity™

Publications

  1. Giorda, R. et al. (1992) Genomic structure and strain-specific expression of the natural killer cell receptor NKR-P1. J. Immunol. 149(6): 1957-1963
  2. Stenström, M. et al. (2005) Natural killer T-cell populations in C57BL/6 and NK1.1 congenic BALB.NK mice-a novel thymic subset defined in BALB.NK mice. Immunology 114(3): 336-345
  3. Ruiz, A. L. et al. (2014)
    NK1.1
    +
    CD8
    +
    T cells escape TGF-β control and contribute to early microbial pathogen response.
    Nat Commun. 5: 5150

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