Clone:
REA859
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
ICFC
Alternative names:
S100-A9, Calgranulin-B, MRP-14, p14

Extended validation for MRP14 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA859
900027-
MAC387-
MRP 1H9++
Cells were incubated with an excess of purified unconjugated MRP14 (REA859) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for MRP14. Human peripheral blood (PBMCs) after erythrocyte lysis were stained with a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with MRP14 antibodies. As a control, MRP14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MRP14. Human peripheral blood (PBMCs) after erythrocyte lysis were stained with a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with MRP14 antibodies. As a control, MRP14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MRP14. Human peripheral blood (PBMCs) after erythrocyte lysis were stained with a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with MRP14 antibodies. As a control, MRP14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for MRP14. Human peripheral blood (PBMCs) after erythrocyte lysis were stained with a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with MRP14 antibodies. As a control, MRP14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for MRP14. Human peripheral blood (PBMCs) after erythrocyte lysis were stained with a suitable counterstaining. Cells were then fixed and permeabilized followed by a staining with MRP14 antibodies. As a control, MRP14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris and cell doublets were excluded from the analysis based on scatter signals. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Specifications for MRP14 Antibody, anti-human, REAfinity™

Overview

Clone REA859 recognizes the human MRP14 antigen, a calcium- and zinc-binding protein also known as S100A9 or calgranulin-B. MRP14 is mainly expressed in myeloid cells, e.g. MDSCs, neutrophils, and monocytes, and plays an important role in the regulation of inflammatory processes and immune response. MRP14 is involved in neutrophil chemotaxis and adhesion, and is able to increase the bactericidal activity of neutrophils.
Additional information: Clone REA859 displays negligible binding to Fc receptors.

Alternative names

S100-A9, Calgranulin-B, MRP-14, p14

Detailed product information

Technical specifications

CloneREA859
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (I), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenMRP14
Alternative names of antigenS100-A9, Calgranulin-B, MRP-14, p14
Molecular mass of antigen [kDa]13
Distribution of antigenmonocytes
Entrez Gene ID6280
RRIDAB_2726683, AB_2726768, AB_2726684, AB_2726769, AB_2726685, AB_2726770, AB_2726686, AB_2726767

Resources for MRP14 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for MRP14 Antibody, anti-human, REAfinity™

Publications

  1. Odink, K. et al. (1987) Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis. Nature 330(6143): 80-82
  2. Rammes, A. et al. (1997) Myeloid-related protein (MRP) 8 and MRP14, calcium-binding proteins of the S100 family, are secreted by activated monocytes via a novel, tubulin-dependent pathway. J. Biol. Chem. 272(14): 9496-9502
  3. Loser, K. et al. (2010)
    The toll-like receptor 4 ligands Mrp8 and Mrp14 are crucial in the development of autoreactive CD8
    +
    T cells.
    Nat Med 16(6): 713-717

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