Tumor necrosis factor alpha (TNF-α) has a broad spectrum of biological activities. In addition to its central role in inflammation TNF-α is involved in regulating apoptotic cell death, cellular proliferation, and differentiation.
MACS GMP Recombinant Human TNF-α is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human TNF-α

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TNF-α. The peak corresponds to the calculated molecular mass of 17353 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human TNF-α. The peak corresponds to the calculated molecular mass of 17353 Da.

Figure 2

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MACS GMP Recombinant Human TNF-α biological activity.
Activity of Human TNF-α, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 2

MACS GMP Recombinant Human TNF-α biological activity.
Activity of Human TNF-α, GMP grade, (blue bar) was compared to other commercially available products (black and grey bar).

Figure 3

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MACS GMP Recombinant Human TNF-α batch-to-batch variability.
Activity of three different production batches of Human MACS GMP TNF-α.

Figure 3

MACS GMP Recombinant Human TNF-α batch-to-batch variability.
Activity of three different production batches of Human MACS GMP TNF-α.

Specifications for
MACS
®
GMP Recombinant Human TNF-α

Overview

Tumor necrosis factor alpha (TNF-α) has a broad spectrum of biological activities. In addition to its central role in inflammation TNF-α is involved in regulating apoptotic cell death, cellular proliferation, and differentiation.
MACS GMP Recombinant Human TNF-α is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

TNF-α is mainly produced by activated monocytes and macrophages, but also by a broad variety of other cell types including lymphoid cells, mast cells, endothelial cells, fibroblasts, and astrocytes. Beside its primary role in immune regulation, TNF-α promotes angiogenesis, bone resorption, and thrombotic processes.

Applications

MACS GMP Recombinant Human TNF-α can be used for a variety of applications, including the
ex vivo
generation of human dendritic cells from enriched CD14
+
monocyte populations.
2–4

Biological activity

  • The specific activity is determined by cytotoxicity assay using L929 cells provided by the German Collection of Microorganisms and Cell Cultures (DSMZ) in the presence of 1 μg/mL actinomycin D according to Baarsch
    et al
    .
    1
    The cytotoxicity assay was calibrated with the international standard for human TNF-α (NIBSC code 88/786) provided by the National Institute for Biological Standards and Control.
  • Minimal specific activity: ≥ 2×
    10
    7
    IU/mg
  • Typical specific activity: ≥ 3.9×
    10
    7
    IU/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human TNF-α

Documents and Protocols

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human TNF-α

Publications

  1. Baarsch, M. J. et al. (1991) Detection of tumor necrosis factor alpha from porcine alveolar macrophages using an L929 fibroblast bioassay. J. Immunol. Methods 140: 15-22
  2. Bender, A. et al. (1996) Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. J. Immunol. Methods 196: 121-135
  3. Romani, N. et al. (1996) Generation of mature dendritic cells from human blood. An improved method with special regard to clinical applicability. J. Immunol. Methods 196: 137-151
  4. Jonuleit et al. (1997) Pro-inflammatory cytokines and prostaglandins induce maturation of potent immunostimulatory dendritic cells under fetal calf serum-free conditions. Eur. J. Immunol. 27: 3135-3142

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®
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