Interleukin 15 (IL-15) is a potent lymphoid cell growth factor and stimulates the proliferation of activated T cells and the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, activation, and proliferation of NK cells.
MACS GMP Recombinant Human IL-15 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Data and images for
MACS
®
GMP Recombinant Human IL-15

Figures

Figure 1

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Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-15. The peak corresponds to the calculated molecular mass of 12770 Da.

Figure 1

Mass spectrometry analysis (ESI-MS) of MACS GMP Recombinant Human IL-15. The peak corresponds to the calculated molecular mass of 12770 Da.

Figure 2

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MACS GMP Recombinant Human IL-15 biological activity.
Activity of Human IL-15, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Figure 2

MACS GMP Recombinant Human IL-15 biological activity.
Activity of Human IL-15, GMP grade, (blue bar) was compared to another commercially available product (black bar).

Figure 3

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MACS GMP Recombinant Human IL-15 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP IL-15.

Figure 3

MACS GMP Recombinant Human IL-15 batch-to-batch variability.
Activity of three different production batches of Human MACS GMP IL-15.

Specifications for
MACS
®
GMP Recombinant Human IL-15

Overview

Interleukin 15 (IL-15) is a potent lymphoid cell growth factor and stimulates the proliferation of activated T cells and the generation of cytotoxic T lymphocytes (CTLs). IL-15 also induces the generation, activation, and proliferation of NK cells.
MACS GMP Recombinant Human IL-15 is designed for
ex vivo
cell culture processing. No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin. The product is lyophilized without carrier protein or preservatives.

Detailed product information

Background information

IL-15 is a member of the four α-helix bundle cytokine family. It is produced by different cell types, including epithelial cells, monocytes, muscle and placenta cells. In addition to its ability to activate T cells and NK cells, IL-15 is also important for B cell growth and immunoglobulin production as well as for the maintenance of CD8
+
memory T cells. For binding and signaling IL-15 uses the unique IL-15 receptor α-chain, but shares the β- and γ-chain of the IL-2 receptor.

Applications

MACS GMP Recombinant Human IL-15 can be used for a variety of applications, including the
ex vivo
generation of cytotoxic T lymphocytes
2,3
or the
ex vivo
stimulation of NK cells
4,5
.

Biological activity

  • The specific activity is determined by proliferation assay according to Soman
    et al.1
    using CTLL-2 cells. The proliferation assay was calibrated with the reference standard for human IL-15 (NIBSC code 95/554) provided by the National Institute for Biological Standards and Control.
  • Specific activity: ≥ 7×
    10
    6
    U/mg

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Disclaimer

MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.

Resources for
MACS
®
GMP Recombinant Human IL-15

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for
MACS
®
GMP Recombinant Human IL-15

Publications

  1. Soman, G. et al. (2009) MTS dye based colorimetric CTLL-2 cell proliferation assay for product release and stability monitoring of interleukin-15: assay qualification, standardization and statistical analysis. J. Immunol. Methods 348: 83-94
  2. Alves et al. (2003)
    IL-15 induces antigen-independent expansion and differentiation of human naive CD8
    +
    T cells
    in vitro
    .
    Blood 102: 2541-2546
  3. Kaneko et al. (2009) IL-7 and IL-15 allow the generation of suicide gene-modified alloreactive self-renewing central memory human T lymphocytes. Blood 113: 1006-1015
  4. Cho, D. and Campana, D. (2009) Expansion and activation of natural killer cells for cancer immunotherapy. Korean J. Lab. Med. 29: 89-96
  5. Siegler, U. et al. (2010) Good manufacturing practice-compliant cell sorting and large-scale expansion of single KIR-positive alloreactive human natural killer cells for multiple infusions to leukemia patients. Cytotherapy 12(6): 750-763

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®
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