Clone:
REA124
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Interferon α/β receptor 2 (IFN-R-2, IFN-α binding protein, IFN-α/β receptor 2), IFN-R, IFN-alpha-REC, IFNABR, IFNARB

Extended validation for IFNAR2 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of IFNAR2 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with IFNAR2-PE (REA124, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of IFNAR2 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with IFNAR2-PE (REA124, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of IFNAR2 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with IFNAR2-PE (REA124, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of human CD45 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45-PE, clone (REAL258). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of human CD45 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD45-PE, clone (REAL258). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for IFNAR2 Antibody, anti-human, REAfinity™

Overview

Clone REA124 recognizes the human interferon α/β receptor 2 (IFNAR2) antigen, a 100 kDa single-pass type I membrane protein that forms one of the two chains of a receptor for the type I interferons IFN-α, IFN-β, and IFN-ω. These interferons are a group of structurally and functionally related proteins, induced by viruses or double-stranded RNA and defined by their ability to establish an antiviral state in cells. IFNAR2 is expressed on most lymphocytes, monocytes, and granulocytes, although IFNAR2 expression is higher on monocytes and granulocytes than on lymphocytes. It is involved in IFN-mediated STAT1, STAT2, and STAT3 activation. The alternatively spliced isoform of IFNAR2 is a potent inhibitor of type I IFN receptor activity.
Additional information: Clone REA124 displays negligible binding to Fc receptors.

Alternative names

Interferon α/β receptor 2 (IFN-R-2, IFN-α binding protein, IFN-α/β receptor 2), IFN-R, IFN-alpha-REC, IFNABR, IFNARB

Detailed product information

Technical specifications

CloneREA124
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenIFNAR2
Alternative names of antigenInterferon α/β receptor 2 (IFN-R-2, IFN-α binding protein, IFN-α/β receptor 2), IFN-R, IFN-alpha-REC, IFNABR, IFNARB
Molecular mass of antigen [kDa]54,8
Distribution of antigenlymphocytes, monocytes, fibroblasts
Entrez Gene ID3455
RRIDAB_2652221, AB_2652222, AB_2652223, AB_2652224, AB_2652225, AB_2652226, AB_2652227, AB_2652228, AB_2652229, AB_2652230

Resources for IFNAR2 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for IFNAR2 Antibody, anti-human, REAfinity™

Publications

  1. Constantinescu, S. N. et al. (1995) Expression and signaling specificity of the IFNAR chain of the type I interferon receptor complex. Proc. Natl. Acad. Sci. U.S.A. 92(23): 10487-10491
  2. Novick, D. et al. (1995) Soluble and membrane-anchored forms of the human IFN-alpha/beta receptor. J. Leukoc. Biol. 57(5): 712-718
  3. Novick, D. et al. (1994) The human interferon alpha/beta receptor: characterization and molecular cloning. Cell 77(3): 391-400

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