Clone:
AC122
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2aκ, mouse IgG2a
Applications:
FC, MICS, IF, IHC, MC
Alternative names:
HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Extended validation for HLA-DR Antibody, anti-human

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-HLA-DR. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-HLA-DR antibodies and plotted against the side scatter. As a control, Anti-HLA-DR antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
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Comparison of staining pattern on non-fixed and fixed cells using HLA-DR (AC122). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
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Comparison of staining pattern on non-fixed and fixed cells using HLA-DR (AC122). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using HLA-DR (AC122). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for HLA-DR Antibody, anti-human

Overview

Anti-HLA-DR antibodies react with the human major histocompatibility (MHC) class II antigen HLA-DR. HLA-DR is constitutively expressed on professional antigen-presenting cells like dendritic cells, B cells, and monocytes/macrophages. Its expression is further up-regulated upon activation. On T cells, NK cells, hematopoietic precursor cells, and some epithelial cells the expression of HLA-DR is induced by cell activation.

Alternative names

HLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5

Detailed product information

Technical specifications

CloneAC122
Clonalitymonoclonal
Isotypemouse IgG2aκ, mouse IgG2a
Isotype controlIsotype Control Antibody, mouse IgG2a
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate, other
Cross-reactivity
rhesus monkey (
Macaca mulatta
)
,
cynomolgus monkey (
Macaca fascicularis
)
,
african green monkey (
Chlorocebus aethiops
)
,
common marmoset (
Callithrix jacchus
)
, guinea pig
AntigenHLA-DR
Alternative names of antigenHLA-DRA, HLA-DRB1, HLA-DRB3, HLA-DRB4, HLA-DRB5
Molecular mass of antigen [kDa]27-33
Entrez Gene ID3123, 3122, 3127, 3126, 3125, 16149
RRIDAB_2726157, AB_2751178, AB_2751111, AB_2733324, AB_2733325, AB_2726435, AB_2726158, AB_2733910, AB_2733911, AB_2733193, AB_2733194, AB_2733091, AB_2733092, AB_2733021, AB_2733022, AB_2726433, AB_2726156, AB_2661330, AB_2726434

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