The Debris Removal Solution is a ready-to-use density gradient reagent. It allows for the fast and effective removal of cell debris from viable cells after dissociation of various tissue types while applying full acceleration and full brake during centrifugation.

Data and images for Debris Removal Solution

Figures

Figure 1

One total adult rat heart was dissociated using the Neonatal Heart Dissociation Kit, mouse and rat in combination with the gentleMACS™ Octo Dissociator with Heaters. Subsequently, debris was depleted using the Debris Removal Solution and erythrocytes were removed according to the Red Blood Cell Lysis protocol described in the Neonatal Heart Dissociation Kit data sheet. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer based on scatter signals to demonstrate absence of debris after debris removal.
After dissociation
After dissociation and debris removal
After dissociation, debris removal, and erythrocyte removal
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Figure 1

One total adult rat heart was dissociated using the Neonatal Heart Dissociation Kit, mouse and rat in combination with the gentleMACS™ Octo Dissociator with Heaters. Subsequently, debris was depleted using the Debris Removal Solution and erythrocytes were removed according to the Red Blood Cell Lysis protocol described in the Neonatal Heart Dissociation Kit data sheet. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer based on scatter signals to demonstrate absence of debris after debris removal.
View details

Figure 1

One total adult rat heart was dissociated using the Neonatal Heart Dissociation Kit, mouse and rat in combination with the gentleMACS™ Octo Dissociator with Heaters. Subsequently, debris was depleted using the Debris Removal Solution and erythrocytes were removed according to the Red Blood Cell Lysis protocol described in the Neonatal Heart Dissociation Kit data sheet. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer based on scatter signals to demonstrate absence of debris after debris removal.
View details

Figure 1

One total adult rat heart was dissociated using the Neonatal Heart Dissociation Kit, mouse and rat in combination with the gentleMACS™ Octo Dissociator with Heaters. Subsequently, debris was depleted using the Debris Removal Solution and erythrocytes were removed according to the Red Blood Cell Lysis protocol described in the Neonatal Heart Dissociation Kit data sheet. Cells were analyzed by flow cytometry using the MACSQuant
®
Analyzer based on scatter signals to demonstrate absence of debris after debris removal.

Figure

View details
Debris removal protocol overview, steps 6–13
Debris removal protocol overview, steps 6–13

Specifications for Debris Removal Solution

Overview

The Debris Removal Solution is a ready-to-use density gradient reagent. It allows for the fast and effective removal of cell debris from viable cells after dissociation of various tissue types while applying full acceleration and full brake during centrifugation.

Detailed product information

Cell debris often occurs after tissue dissociation and impairs downstream applications. The Debris Removal Solution allows for the fast and effective removal of cell debris after tissue dissociation for high quality downstream applications, including cell separation, flow analysis, and cell culture. It has been particularly tested for debris removal from dissociated rat and mouse heart tissue and human and mouse tumor tissue.

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Debris Removal Solution

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