Clone:
REA1242
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
ADGRE5, TM7LN1, EMR1

Extended validation for CD97 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
WT
KO
View details
Fluorescence microscopy image of CD97 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD97-PE (REA1242, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Fluorescence microscopy image of CD97 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD97-PE (REA1242, red) and counterstained with DRAQ5 (blue) as DNA stain.
Fluorescence microscopy image of CD97 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD97-PE (REA1242, red) and counterstained with DRAQ5 (blue) as DNA stain.
View details
Overlay histogram showing flow cytometric analysis of CD97 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD97-PE, clone (REA1242). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD97 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD97-PE, clone (REA1242). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD97 Antibody, anti-human, REAfinity™

Overview

Clone REA1242 reacts with the human CD97 antigen, a member of the epidermal growth factor (EGF)-TM7 family of cell surface receptors. CD97 is expressed by various cell types of the hematopoietic system and also on smooth muscle cells. Highest constitutive expression is found on granulocytes and monocytes. Lymphocytes express CD97 at lower level (T and NK cells expressing higher levels than B cells) but on T cells CD97 is upregulated upon activation via CD3 and CD28 or PHA. CD97 is a ligand for CD55 (decay-accelerating factor, DAF) and has been shown to mediate cell adhesion and co-stimulation of T cell proliferation.
Additional information: Clone REA1242 displays negligible binding to Fc receptors.

Alternative names

ADGRE5, TM7LN1, EMR1

Detailed product information

Technical specifications

CloneREA1242
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD97
Alternative names of antigenADGRE5, TM7LN1, EMR1
Molecular mass of antigen [kDa]90
Distribution of antigengranulocytes, monocytes, B cells, T cells, NK cells, smooth muscle
Entrez Gene ID976
RRIDAB_2819726, AB_2819731, AB_2819727, AB_2819732, AB_2819728, AB_2819733, AB_2819729, AB_2819730

Resources for CD97 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD97 Antibody, anti-human, REAfinity™

Publications

  1. Hamann, J. et al. (1995) Expression cloning and chromosomal mapping of the leukocyte activation antigen CD97, a new seven-span transmembrane molecule of the secretion receptor superfamily with an unusual extracellular domain. J. Immunol. 155(4): 1942-1950

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