Clone:
REA195
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
TACTILE

Extended validation for CD96 (TACTILE) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA195
NK92.39++
Cells were incubated with an excess of purified unconjugated CD96 (TACTILE) (REA195) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD96 (TACTILE). Human peripheral blood mononuclear cells (PBMCs) were stained with CD96 (TACTILE) antibodies and with a suitable counterstaining. As a control, CD96 (TACTILE) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD96 (TACTILE). Human peripheral blood mononuclear cells (PBMCs) were stained with CD96 (TACTILE) antibodies and with a suitable counterstaining. As a control, CD96 (TACTILE) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD96 (TACTILE). Human peripheral blood mononuclear cells (PBMCs) were stained with CD96 (TACTILE) antibodies and with a suitable counterstaining. As a control, CD96 (TACTILE) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD96 (TACTILE). Human peripheral blood mononuclear cells (PBMCs) were stained with CD96 (TACTILE) antibodies and with a suitable counterstaining. As a control, CD96 (TACTILE) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD96 (TACTILE) (REA195). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD96 (TACTILE) (REA195). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD96 (TACTILE) (REA195). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD96 (TACTILE) Antibody, anti-human, REAfinity™

Overview

Clone REA195 recognizes CD96, which is also known as T cell–activated increased late expression (TACTILE). CD96 is a single-pass transmembrane glycoprotein belonging to the Ig superfamily and consists of three extracellular Ig-like domains and a membrane proximal region rich in proline/serine/threonine. Expression of CD96 is found on T cells, NK cells, and on majority of CD34
+
CD38
-
acute myeloid leukemic (AML) cells. CD96 interacts with CD155, which is also known as polio virus receptor and promotes NK cell adhesion to target cells expressing CD155. CD96 is further described as a tumor marker for T cell–acute lymphoblastic leukemia (T-ALL) and AML.
Additional information: Clone REA195 displays negligible binding to Fc receptors.

Alternative names

TACTILE

Detailed product information

Technical specifications

CloneREA195
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD96 (TACTILE)
Alternative names of antigenTACTILE
Distribution of antigenT cells, NK cells
RRIDAB_2751863, AB_2751832, AB_2784326, AB_2784325, AB_2659656, AB_2659657, AB_2659662, AB_2659663

Resources for CD96 (TACTILE) Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD96 (TACTILE) Antibody, anti-human, REAfinity™

Publications

  1. Fuchs, A. et al. (2004) Cutting Edge: CD96 (Tactile) promotes NK cell-target cell adhesion by interacting with the poliovirus receptor (CD155). J. Immunol. 172: 3994-3998
  2. Meyer, D. et al. (2009) CD96 interaction with CD155 via its first Ig-like domain is modulated by alternative splicing or mutations in distal Ig-like domains. J. Biol. Chem. 284: 2235-2244

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