Clone:
REA968
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MC
Alternative names:
B7-2, B70, CD28LG2, LAB72

Extended validation for CD86 Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD86. Human peripheral blood mononuclear cells (PBMCs) were stained with CD86 antibodies and with a suitable counterstaining. As a control, CD86 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD86 (REA968). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD86 (REA968). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD86 (REA968). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD86 Antibody, anti-human, REAfinity™

Overview

Clone REA968 recognizes the human CD86 antigen, also known as B7-2 or B70, which is an 80 kDa molecule and a member of the immunoglobulin superfamily. Together with CD80 (B7-1) it belongs to the B7 family of co-stimulatory molecules. CD86 is expressed on activated B and T cells, dendritic cells, and monocytes/macrophages. The interaction of CD86 with its ligands CD28 and CD152 (CTLA- 4) plays a critical role in induction and regulation of immune responses, e.g., cross-talk between T and B cells, T cell costimulation, or immunoglobulin class-switching. Binding of CD86 to CD28 on T cells results in transduction of costimulatory signals for activation or proliferation of T cells or cytokine production.
Additional information: Clone REA968 displays negligible binding to Fc receptors.

Alternative names

B7-2, B70, CD28LG2, LAB72

Detailed product information

Technical specifications

CloneREA968
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD86
Alternative names of antigenB7-2, B70, CD28LG2, LAB72
Molecular mass of antigen [kDa]35
Distribution of antigenB cells, dendritic cells, Langerhans cells, macrophages, monocytes, T cells, B cell subsets, Langerhans cells
Entrez Gene ID942
RRIDAB_2727371, AB_2727436, AB_2727372, AB_2727437, AB_2727373, AB_2727441, AB_2727378, AB_2727442, AB_2727379, AB_2727438, AB_2727374, AB_2727375, AB_2727439, AB_2727376, AB_2727440, AB_2727377, AB_2727434, AB_2727370, AB_2801913, AB_2727435

References for CD86 Antibody, anti-human, REAfinity™

Publications

  1. Azuma, M. et al. (1993) B70 antigen is a second ligand for CTLA-4 and CD28. Nature 366(6450): 76-79
  2. Engel, P. et al. (1994) The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood 84(5): 1402-1407
  3. Lanier, L. L. et al. (1995) CD80 (B7) and CD86 (B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL. J. Immunol. 154(1): 97-105