Clone:
REA100
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
LILRA4, ILT7

Extended validation for CD85g (ILT7) Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA100
17G10.2++
Cells were incubated with an excess of purified unconjugated CD85g (ILT7) (REA100) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD85g (ILT7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85g (ILT7) antibodies and with a suitable counterstaining. As a control, CD85g (ILT7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD85g (ILT7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85g (ILT7) antibodies and with a suitable counterstaining. As a control, CD85g (ILT7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD85g (ILT7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85g (ILT7) antibodies and with a suitable counterstaining. As a control, CD85g (ILT7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD85g (ILT7). Human peripheral blood mononuclear cells (PBMCs) were stained with CD85g (ILT7) antibodies and with a suitable counterstaining. As a control, CD85g (ILT7) antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD85g (ILT7) (REA100). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD85g (ILT7) (REA100). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD85g (ILT7) (REA100). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD85g (ILT7) Antibody, anti-human, REAfinity™

Overview

The clone REA100 recognizes human CD85g, also known as immunoglobulin-like transcript 7 (ILT7), a cell surface receptor which is specifically expressed on human plasmacytoid dendritic cells (PDCs). ILT7 associates with FcεR1γ chain through positively charged arginine residue located in the transmembrane domain to form a receptor complex. Cross-linking of ILT7 results in FcεR1γ-dependent activation of an ITAM-mediated signaling pathway, which negatively influences TLR7/9 induced type I IFN production. Cross-linking of ILT7 by REA100 monoclonal antibodies inhibits type I IFN production in human PDCs.
Additional information: Clone REA100 display negligible binding to Fc receptors.

Alternative names

LILRA4, ILT7

Detailed product information

Technical specifications

CloneREA100
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD85g (ILT7)
Alternative names of antigenLILRA4, ILT7
Molecular mass of antigen [kDa]53
Distribution of antigendendritic cells, macrophages, monocytes, NK cells, T cells, lymphocytes, eosinophils, neutrophils
Entrez Gene ID23547
RRIDAB_2659354, AB_2659355, AB_2659356, AB_2659357, AB_2659358, AB_2659359, AB_2659360, AB_2659361

References for CD85g (ILT7) Antibody, anti-human, REAfinity™

Publications

  1. Cao, W. et al. (2006)
    Plasmacytoid
    dendritic cell–specific receptor ILT7–FcεRIγ inhibits Toll-like receptor–induced interferon production.
    J. Exp. Med. 203: 1399-1405

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