Clone:
REA1102
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
Ly9b, SLAMF5, hCD84, GR6

Extended validation for CD84 Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA1102
CD84.1.21-
MZ18-21F6++
2G7++
Cells were incubated with an excess of purified unconjugated CD84 (REA1102) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for Anti-FCeRIa. Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-FCeRIa antibodies and with a suitable counterstaining. As a control, Anti-FCeRIa antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD84 (REA1102). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD84 (REA1102). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD84 (REA1102). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD84 Antibody, anti-human, REAfinity™

Overview

Clone REA1102 recognizes the human CD84 antigen, a 65–80 kDa single-chain surface glycoprotein and member of the CD2 subset of the immunoglobulin (Ig) superfamily. CD84 is expressed on granulocytes, monocytes, macrophages, B cells, platelets, antigen-presenting cells, and subsets of T cells including thymocytes and mature T cells. Homophilic ligation of CD84 or ligation with CD84 antibodies has been shown to lead to enhanced proliferation of T cells as well as IFN-γ secretion.
Additional information: Clone REA1102 displays negligible binding to Fc receptors.

Alternative names

Ly9b, SLAMF5, hCD84, GR6

Detailed product information

Technical specifications

CloneREA1102
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD84
Alternative names of antigenLy9b, SLAMF5, hCD84, GR6
Molecular mass of antigen [kDa]36
Distribution of antigenB cells, granulocytes, macrophages, monocytes, platelets, T cells, antigen-presenting cells, thymocytes
Entrez Gene ID8832
RRIDAB_2751611, AB_2751660, AB_2751612, AB_2751661, AB_2751613, AB_2751662, AB_2751614, AB_2751663, AB_2751615, AB_2751664, AB_2751616, AB_2751659

Resources for CD84 Antibody, anti-human, REAfinity™

Certificates

Please follow this
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to search for Certificates of Analysis (CoA) by lot number.

References for CD84 Antibody, anti-human, REAfinity™

Publications

  1. de la Fuente, M. A. et al. (1997) CD84 leukocyte antigen is a new member of the Ig superfamily. Blood 90: 2398-2405
  2. Martin, M. et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-γ secretion: adhesion is mediated by Ig-like domain 1. J. Immunol. 167: 3668-3676
  3. Tangye, S. G. et al. (2003) Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation. J. Immunol. 171: 2485-2495
  4. Zaiss, M. et al. (2003) CD84 expression on human hematopoietic progenitor cells. Exp. Hematol. 31: 798-805

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