Clone:
REA231
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
CD72b, Lyb-2

Extended validation for CD72 Antibody, anti-human, REAfinity™

Specificity

Knockout validation
To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.
View details
Overlay histogram showing flow cytometric analysis of CD72 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD72-PE, clone (REA231). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Overlay histogram showing flow cytometric analysis of CD72 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD72-PE, clone (REA231). Flow cytometry was performed with the MACSQuant
®
Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD72 Antibody, anti-human, REAfinity™

Overview

Clone REA231 recognizes CD72, a 45 kDa type II membrane protein with a C-type lectin-like domain. Expression of CD72 is found on most B-lineage cells, follicular dendritic cells, mast cells, and liver Kupffer cells. CD72 is recognized as negative regulator of B cell receptor (BCR) signaling, as well as regulating mast cell growth and differentiation via KIT activation. The inhibitory role of CD72 is exerted via the ITIM sequence in the cytoplasmic tail.
Additional information: Clone REA231 displays negligible binding to Fc receptors.

Alternative names

CD72b, Lyb-2

Detailed product information

Technical specifications

CloneREA231
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hostcell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD72
Alternative names of antigenCD72b, Lyb-2
Distribution of antigenB cells, mast cells
RRIDAB_2659139, AB_2659140, AB_2659141, AB_2659142, AB_2659143, AB_2659144, AB_2659145, AB_2659146, AB_2659147, AB_2659148, AB_2659149, AB_2659150, AB_2659151, AB_2659138

Resources for CD72 Antibody, anti-human, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD72 Antibody, anti-human, REAfinity™

Publications

  1. Kataoka, T. R. et al. (2010) CD72 negatively regulates KIT-mediated responses in human mast cells. J. Immunol. 184: 2468-2475
  2. Li, D. H. et al. (2006) CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes. J. Immunol. 176: 5321-5328

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