Clone:
Y1/82A
Type of antibody:
Primary antibodies
Isotype:
mouse IgG2bκ
Applications:
ICFC, MICS, IF, IHC
Alternative names:
gp110, Lamp4, SCARD1, macrosialin

Extended validation for CD68 Antibody, anti-human

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with Y1/82A
REA886++
Cells were incubated with an excess of purified unconjugated CD68 (Y1/82A) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Specifications for CD68 Antibody, anti-human

Overview

The monoclonal antibody Y1/82A reacts with CD68, a 110 kDa type I transmembrane glycoprotein classified as a lysosomal/endosomal-associated membrane glycoprotein (LAMP) family member. CD68 is highly expressed by human monocytes and tissue macrophages but also found in subpopulations of B cells, fibroblasts, endothelial, and other cells. It is found predominantely in late endosomal and lysosomal compartments, with a modest level of cell surface expression. CD68 is suggested to be involved in phagocytic activities of tissue macrophages, in intracellular lysosomal metabolism, and extracellular cell-cell and cell-pathogen interactions.

Alternative names

gp110, Lamp4, SCARD1, macrosialin

Detailed product information

Technical specifications

CloneY1/82A
Clonalitymonoclonal
Isotypemouse IgG2bκ
Isotype controlIsotype Control Antibody, mouse IgG2b
Hostmouse
Type of antibodyPrimary antibodies
Specieshuman, non-human primate
Cross-reactivity
cynomolgus monkey (
Macaca fascicularis
)
AntigenCD68
Alternative names of antigengp110, Lamp4, SCARD1, macrosialin
Molecular mass of antigen [kDa]35
Distribution of antigenB cells, dendritic cells, fibroblasts, Langerhans cells, macrophages, mast cells, monocytes, osteoclasts, platelets, T cells, lymphocytes, basophils, eosinophils, liver
Entrez Gene ID968
RRIDAB_2784269, AB_2659041, AB_2659042, AB_10829617, AB_2784270

References for CD68 Antibody, anti-human

Publications

  1. Holness, C. L. and Simmons, D. L. (1993) Molecular cloning of CD68, a human macrophage marker related to lysosomal glycoproteins. Blood 81: 1607-1613
  2. Kurushima, H. et al. (2000) Surface expression and rapid internalization of macrosialin (mouse CD68) on elicited mouse peritoneal macrophages. J. Leukoc. Biol. 67: 104-108
  3. Saito, N. et al. (1991) Ultrastructural localization of the CD68 macrophage-associated antigen in human blood neutrophils and monocytes. Am. J. Pathol. 139: 1053-1059
  4. Holness, C. L. et al. (1993) Macrosialin, a mouse macrophage-restricted glycoprotein, is a member of the lamp/lgp family. J. Biol. Chem. 268: 9661-9666
  5. Gottfried, L. A. et al. (2008) Expression of CD68 in non-myeloid cell types. Scand. J. Immunol. 67: 453-463

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